Vpx association with mature core structures of HIV-2

被引：50

作者：

Kewalramani, VN

Emerman, M

机构：

[1] FRED HUTCHINSON CANC RES CTR,DIV MOLEC MED,SEATTLE,WA 98104

[2] FRED HUTCHINSON CANC RES CTR,DIV BASIC SCI,SEATTLE,WA 98104

[3] UNIV WASHINGTON,PROGRAM MOLEC & CELLULAR BIOL,SEATTLE,WA 98195

[4] UNIV WASHINGTON,DEPT MICROBIOL,SEATTLE,WA 98195

来源：

VIROLOGY | 1996年 / 218卷 / 01期

关键词：

D O I：

10.1006/viro.1996.0176

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Vpx is a virion-incorporated protein encoded by HIV-2 and certain strains of SIV which is necessary for efficient infection of lymphocytes and macrophages. To determine the location of Vpx within the HIV-2 particle, core structures were obtained from mature HIV-2 virions. Analysis of purified cores reveal the presence of monomeric Vpx as well as a nondenaturable and nonreducible higher order Vpx complex. Characterization of the core-associated Vpx complex suggests it to be a Vpx homodimer, indicative of high concentrations of Vpx. The incorporation and concentration of Vpx in HIV-2 cores support the hypothesis that it functions within the viral preintegration complex. (C) 1996 Academic Press, Inc.

引用

收藏

页码：159 / 168

页数：10

相关论文

共 47 条

[1] A RECOMMENDATION FOR VISUALIZING DISULFIDE BONDING BY ONE-DIMENSIONAL SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

ALLORE, RJ ;

BARBER, BH .

ANALYTICAL BIOCHEMISTRY, 1984, 137 (02) :523-527

[2] DISTINCT EFFECTS IN PRIMARY MACROPHAGES AND LYMPHOCYTES OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 ACCESSORY GENES VPR, VPU, AND NEF - MUTATIONAL ANALYSIS OF A PRIMARY HIV-1 ISOLATE [J].

BALLIET, JW ;

KOLSON, DL ;

EIGER, G ;

KIM, FM ;

MCGANN, KA ;

SRINIVASAN, A ;

COLLMAN, R .

VIROLOGY, 1994, 200 (02) :623-631

[3] EQUINE INFECTIOUS-ANEMIA VIRUS AND HUMAN-IMMUNODEFICIENCY-VIRUS DNA-SYNTHESIS INVITRO - CHARACTERIZATION OF THE ENDOGENOUS REVERSE-TRANSCRIPTASE REACTION [J].

BORROTOESODA, K ;

BOONE, LR .

JOURNAL OF VIROLOGY, 1991, 65 (04) :1952-1959

[4] A NUCLEOPROTEIN COMPLEX MEDIATES THE INTEGRATION OF RETROVIRAL DNA [J].

BOWERMAN, B ;

BROWN, PO ;

BISHOP, JM ;

VARMUS, HE .

GENES & DEVELOPMENT, 1989, 3 (04) :469-478

[5] A NUCLEAR-LOCALIZATION SIGNAL WITHIN HIV-1 MATRIX PROTEIN THAT GOVERNS INFECTION OF NONDIVIDING CELLS [J].

BUKRINSKY, MI ;

HAGGERTY, S ;

DEMPSEY, MP ;

SHAROVA, N ;

ADZHUBEI, A ;

SPITZ, L ;

LEWIS, P ;

GOLDFARB, D ;

EMERMAN, M ;

STEVENSON, M .

NATURE, 1993, 365 (6447) :666-669

[6] ASSOCIATION OF INTEGRASE, MATRIX, AND REVERSE-TRANSCRIPTASE ANTIGENS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 WITH VIRAL NUCLEIC-ACIDS FOLLOWING ACUTE INFECTION [J].

BUKRINSKY, MI ;

SHAROVA, N ;

MCDONALD, TL ;

PUSHKARSKAYA, T ;

TARPLEY, WG ;

STEVENSON, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (13) :6125-6129

[7] HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].

CHEN, C ;

OKAYAMA, H .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752

[8] COMPARISON OF ROUS SARCOMA VIRUS-SPECIFIC DEOXYRIBONUCLEIC ACID POLYMERASES IN VIRIONS OF ROUS SARCOMA VIRUS AND ROUS SARCOMA VIRUS-INFECTED CHICKEN CELLS [J].

COFFIN, JM ;

TEMIN, HM .

JOURNAL OF VIROLOGY, 1971, 7 (05) :625-&

[9] HUMAN-IMMUNODEFICIENCY-VIRUS VPR PRODUCT IS A VIRION-ASSOCIATED REGULATORY PROTEIN [J].

COHEN, EA ;

DEHNI, G ;

SODROSKI, JG ;

HASELTINE, WA .

JOURNAL OF VIROLOGY, 1990, 64 (06) :3097-3099

[10] VPR IS REQUIRED FOR EFFICIENT REPLICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 IN MONONUCLEAR PHAGOCYTES [J].

CONNOR, RI ;

CHEN, BK ;

CHOE, S ;

LANDAU, NR .

VIROLOGY, 1995, 206 (02) :935-944

← 1 2 3 4 5 →