Structure determination of an exopolysaccharide from an alkaliphilic bacterium closely related to Bacillus spp.

被引：21

作者：

Corsaro, MM

Grant, WD

Grant, S

Marciano, CE

Parrilli, M

机构：

[1] Univ Naples Federico II, Dipartimento Chim Organ & Biol, I-80134 Naples, Italy

[2] Univ Leicester, Dept Microbiol & Immunol, Leicester LE1 7RH, Leics, England

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1999年 / 264卷 / 02期

关键词：

alkaliphilic; Bacillus; exopolysaccharide; glycine; phylogenetic analysis;

D O I：

10.1046/j.1432-1327.1999.00672.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An exopolysaccharide obtained from an alkaliphilic bacterium closely related to Bacillus spp. was found to contain D-galactopyranuronic acid (GalpA), 2,4-diacetamido-2,4,6-trideoxy-D-glucopyranose (QuipNAc4NAc), 2-acetamido-2-deoxy D-mannopyranuronic acid (ManpNAcA) and one uncommon unit of D-galactopyranuronic acid with the carboxyl group amide-linked to glycine [GalpA(Gly)]. The polysaccharide was studied by one-dimensional and two-dimensional H-1-NMR and C-13-NMR spectroscopy both on native polysaccharide and on monosaccharides and oligosaccharides obtained from methanolysis and from anhydrous HF solvolysis, The following linear structure of the repeating unit was established: -->3)-alpha-D-GalpA(Gly)-( 1-->4)-beta-D-ManpNAcA-(1-->4)-alpha-D-GalpA-(1-->3)-alpha-D-QuipNAc4NAc-(1-->. A preliminary phylogenetic assignment for the bacterium is also reported.

引用

页码：554 / 561

页数：8

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