In organello footprinting analysis of rat mitochondrial DNA: Protein interaction upstream of the Ori-L

被引：3

作者：

Cingolani, G ^{[1
]}

Capaccio, L ^{[1
]}

DElia, D ^{[1
]}

Gadaleta, G ^{[1
]}

机构：

[1] UNIV BARI,DEPT BIOCHEM & MOL BIOL,BARI,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 231卷 / 03期

关键词：

D O I：

10.1006/bbrc.1997.6203

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An in organella footprinting approach has been used to probe a protein-DNA interaction of a nuclear coded 25 kDa protein, previously isolated in our laboratory, that binds ''in vitro'' a region within the ND2 gene, located upstream of the Ori-L. Footprinting studies with the purine-modifying reagent dimethyl sulfate and the pirimidine-modifying reagent potassium permanganate were carried out in isolated mitochondria from rat liver. Dimethyl sulfate footprinting has allowed the detection of a protein-DNA interaction within the curved ND2 region with contact sites located in both the strands. Potassium permanganate footprinting allowed detection of an adjacent permanganate-reactive region. We hypothesize that the permanganate-reactive region is a single stranded DNA due to a profound helix distortion induced by a 25 kDa protein binding to the nearest region. (C) 1997 Academic Press.

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页码：856 / 860

页数：5

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