Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis

被引：176

作者：

Euler, Milena ^{[1
]}

Wang, Yongjie ^{[2
]}

Otto, Peter ^{[3
]}

Tomaso, Herbert ^{[3
]}

Escudero, Raquel ^{[4
]}

Anda, Pedro ^{[4
]}

Hufert, Frank T. ^{[1
]}

Weidmann, Manfred ^{[1
]}

机构：

[1] Univ Med Ctr, Dept Virol, Gottingen, Germany

[2] Shanghai Ocean Univ, Lab Marine & Food Microbiol, Coll Food Sci & Technol, Shanghai, Peoples R China

[3] Friedrich Loeffler Inst, Jena, Germany

[4] Inst Salud Carlos III, Lab Espiroquetas & Patogenos Especiales, Serv Bacteriol, Ctr Nacl Microbiol, Madrid, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 07期

关键词：

CHRONIC-GRANULOMATOUS-DISEASE; ISOTHERMAL DNA AMPLIFICATION; REAL-TIME PCR; PHILOMIRAGIA SEPSIS; STRATEGIES; TULAREMIA; SPECIMENS;

D O I：

10.1128/JCM.06504-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Several real-time PCR approaches to develop field detection for Francisella tularensis, the infectious agent causing tularemia, have been explored. We report the development of a novel qualitative real-time isothermal recombinase polymerase amplification (RPA) assay for use on a small ESEQuant Tube Scanner device. The analytical sensitivity and specificity were tested using a plasmid standard and DNA extracts from infected rabbit tissues. The assay showed a performance comparable to real-time PCR but reduced the assay time to 10 min. The rapid RPA method has great application potential for field use or point-of-care diagnostics.

引用

页码：2234 / 2238

页数：5