Unnatural amino acid derived FRET cassettes, terminators and their DNA sequencing potential

被引：13

作者：

Nampalli, S

Zhang, WH

Rao, TS

Xiao, HG

Kotra, LP

Kumar, S

机构：

[1] Amersham Biosci, Piscataway, NJ 08855 USA

[2] Univ Toronto, Fac Pharm, Toronto, ON M5S 2S2, Canada

来源：

TETRAHEDRON LETTERS | 2002年 / 43卷 / 11期

关键词：

D O I：

10.1016/S0040-4039(02)00196-X

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

An unnatural, tri-functional amino acid. t-BOC-L-para-amino-phenylalanine (1). was utilized in the design and syntheses of highly efficient FRET cassettes (4-7) and converted them into 2'.3'-dideoxynucleotide terminators (8-11) for investigating DNA sequencing potential coupled with a thermostable DNA polymerase. Thermo Sequenase(TM) II . (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：1999 / 2003

页数：5

相关论文

共 11 条

[1]

Hobbs F. W., 1991, US patent, Patent No. 5047519

[2] Cassette labeling for facile construction of energy transfer fluorescent primers [J].

Ju, JY ;

Glazer, AN ;

Mathies, RA .

NUCLEIC ACIDS RESEARCH, 1996, 24 (06) :1144-1148

[3] DESIGN AND SYNTHESIS OF FLUORESCENCE ENERGY-TRANSFER DYE-LABELED PRIMERS AND THEIR APPLICATION FOR DNA-SEQUENCING AND ANALYSIS [J].

JU, JY ;

KHETERPAL, I ;

SCHERER, JR ;

RUAN, CC ;

FULLER, CW ;

GLAZER, AN ;

MATHIES, RA .

ANALYTICAL BIOCHEMISTRY, 1995, 231 (01) :131-140

[4] New energy transfer dyes for DNA sequencing [J].

Lee, LG ;

Spurgeon, SL ;

Heiner, CR ;

Benson, SC ;

Rosenblum, BB ;

Menchen, SM ;

Graham, RJ ;

Constantinescu, A ;

Upadhya, KG ;

Cassel, JM .

NUCLEIC ACIDS RESEARCH, 1997, 25 (14) :2816-2822

[5] Electrophoretically uniform fluorescent dyes for automated DNA sequencing [J].

Metzker, ML ;

Lu, J ;

Gibbs, RA .

SCIENCE, 1996, 271 (5254) :1420-1422

[6] Fluorescence resonance energy transfer terminators for DNA sequencing [J].

Nampalli, S ;

Khot, M ;

Kumar, S .

TETRAHEDRON LETTERS, 2000, 41 (46) :8867-8871

[7] Four color fret dye nucleotide terminators for DNA sequencing [J].

Rao, TS ;

Nampalli, S ;

Lavrenov, K ;

Zhang, WH ;

Xiao, HG ;

Nelson, J ;

Kumar, S .

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2001, 20 (4-7) :673-676

[8] New dye-labeled terminators for improved DNA sequencing patterns [J].

Rosenblum, BB ;

Lee, LG ;

Spurgeon, SL ;

Khan, SH ;

Menchen, SM ;

Heiner, CR ;

Chen, SM .

NUCLEIC ACIDS RESEARCH, 1997, 25 (22) :4500-4504

[9] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[10]

TABOR S, 1990, J BIOL CHEM, V265, P8322