Novel Bacillus thuringiensis binary insecticidal crystal proteins active on western corn rootworm, Diabrotica virgifera virgifera LeConte

被引：113

作者：

Ellis, RT ^{[1
]}

Stockhoff, BA ^{[1
]}

Stamp, L ^{[1
]}

Schnepf, HE ^{[1
]}

Schwab, GE ^{[1
]}

Knuth, M ^{[1
]}

Russell, J ^{[1
]}

Cardineau, GA ^{[1
]}

Narva, KE ^{[1
]}

机构：

[1] Dow Agrosci, San Diego, CA 92121 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2002年 / 68卷 / 03期

关键词：

D O I：

10.1128/AEM.68.3.1137-1145.2002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new family of insecticidal crystal proteins was discovered by screening sporulated Bacillus thuringiensis cultures for oral activity against western corn rootworm (WCR) larvae. B. thuringiensis isolates PS80JJ1, PS149B1, and PS167H2 have WCR insecticidal activity attributable to parasporal inclusion bodies containing proteins with molecular masses of ca. 14 and 44 kDa. The genes encoding these polypeptides reside in apparent operons, and the 14-kDa protein open reading frame (ORF) precedes the 44-kDa protein ORF. Mutagenesis of either gene in the apparent operons dramatically reduced insecticidal activity of the corresponding recombinant B. thuringiensis strain. Bioassays performed with separately expressed, biochemically purified 14- and 44-kDa polypeptides also demonstrated that both proteins are required for WCR mortality. Sequence comparisons with other known B. thuringiensis insecticidal proteins failed to reveal homology with previously described Cry, Cyt, or Vip proteins. However, there is evidence that the 44-kDa polypeptide and the 41.9- and 51.4-kDa binary dipteran insecticidal proteins from Bacillus sphaericus are evolutionarily related. The 14- and 44-kDa polypeptides from isolates PS80JJ1, PS149B1, and PS167H2 have been designated Cry34Aa1, Cry34Ab1, and Cry34Ac1, respectively, and the 44-kDa polypeptides from these isolates have been designated Cry35Aa1, Cry35Ab1, and Cry35Ac1, respectively.

引用

页码：1137 / 1145

页数：9

共 44 条

[1] HOW DOES BACILLUS-THURINGIENSIS PRODUCE SO MUCH INSECTICIDAL CRYSTAL PROTEIN [J].