DNA Adenine Methylation of sams1 Gene in Symbiont-Bearing Amoeba proteus

被引：5

作者：

Jeon, Taeck J. ^{[1
]}

机构：

[1] Chosun Univ, Coll Nat Sci, Dept Biol, Kwangju 501759, South Korea

来源：

JOURNAL OF MICROBIOLOGY | 2008年 / 46卷 / 05期

关键词：

endosymbiosis; S-adenosylmethionine; gene regulation; symbiogenesis;

D O I：

10.1007/s12275-008-0129-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The expression of amoeba sams genes is switched from sams1 to sams2 when amoebae are infected with Legionella jeonii. To elucidate the mechanism for the inactivation of host sams1 gene by endosymbiotic bacteria, methylation states of the sams1 gene of D and xD amoebae was compared in this study. The sams1 gene of amoebae was methylated at an internal adenine residue of GATC site in symbiont-bearing xD amoebae but not in symbiont-free D amoebae, suggesting that the modification might have caused the inactivation of sams1 in xD amoebae. The sams1 gene of xD amoebae was inactivated at the transcriptional level. Analysis of DNA showed that adenine residues in L. jeonii sams were also methylated, implying that L. jeonii bacteria belong to a Dam methylase-positive strain. In addition, both SAM and Met appeared to act as negative regulators for the expression of sams1 whereas the expression of sams2 was not affected in amoebae.

引用

页码：564 / 570

页数：7

共 31 条

[21] Role of S-adenosyl-L-methionine in liver health and injury [J].

Mato, Jose M. ;

Lu, Shelly C. .

HEPATOLOGY, 2007, 45 (05) :1306-1312

[22] Myosin dynamics iri live Dictyostelium cells [J].

Moores, SL ;

Sabry, JH ;

Spudich, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (01) :443-446

[23] Characterization of myosin heavy chain and its gene in Amoeba proteus [J].

Oh, SW ;

Jeon, KW .

JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1998, 45 (06) :600-605

[24] Genome-wide analysis of deoxyadenosine methyltransferase-mediated control of gene expression in Escherichia coli [J].

Oshima, T ;

Wada, C ;

Kawagoe, Y ;

Ara, T ;

Maeda, M ;

Masuda, Y ;

Hiraga, S ;

Mori, H .

MOLECULAR MICROBIOLOGY, 2002, 45 (03) :673-695

[25] The s29x gene of symbiotic bacteria in Amoeba proteus with a novel promoter [J].

Pak, JW ;

Jeon, KW .

GENE, 1996, 171 (01) :89-93

[26]

Pak JW, 1997, J EUKARYOT MICROBIOL, V44, P614

[27] Phylogenetic characterization of Legionella-like endosymbiotic X-bacteria in Amoeba proteus:: a proposal for 'Candidatus Legionella jeonii' sp nov [J].

Park, M ;

Yun, ST ;

Kim, MS ;

Chun, J ;

Ahn, TI .

ENVIRONMENTAL MICROBIOLOGY, 2004, 6 (12) :1252-1263

[28] A HIGH-EFFICIENCY HELA-CELL NUCLEAR TRANSCRIPTION EXTRACT [J].

SHAPIRO, DJ ;

SHARP, PA ;

WAHLI, WW ;

KELLER, MJ .

DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1988, 7 (01) :47-55

[29] Metabolism of sulfur amino acids in Saccharomyces cerevisiae [J].

Thomas, D ;

SurdinKerjan, Y .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1997, 61 (04) :503-&

[30] DNA methylation and epigenetics [J].

Vanyushin, B. F. .

RUSSIAN JOURNAL OF GENETICS, 2006, 42 (09) :985-997

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