Brain banks: benefits, limitations and cautions concerning the use of post-mortem brain tissue for molecular studies

被引:130
作者
Ferrer, Isidre [1 ,2 ]
Martinez, Anna [1 ,2 ]
Boluda, Susana [1 ,2 ]
Parchi, Piero [3 ]
Barrachina, Marta [1 ,2 ]
机构
[1] Univ Barcelona, Hosp Univ Bellvitge, IDIBELL, Inst Neuropatol,Serv Anat Patol, Lhospitalet De Llobregat 08907, Spain
[2] CIBERNED, Barcelona, Spain
[3] Univ Bologna, Dipartimento Sci Neurol, Bologna, Italy
关键词
DNA; RNA; protein; post-mortem delay; brain bank; oxidation; epigenetics;
D O I
10.1007/s10561-008-9077-0
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Brain banks are facilities providing an interface between generous donation of nervous tissues and research laboratories devoted to increase our understanding of the diseases of the nervous system, discover new diagnostic targets, and develop new strategies. Considering this crucial role, it is important to learn about the suitabilities, limitations and proper handling of individual brain samples for particular studies. Several factors may interfere with preservation of DNA, RNA, proteins and lipids, and, therefore, special care must be taken first to detect sub-optimally preserved tissues and second to provide adequate material for each specific purpose. Basic aspects related with DNA, RNA and protein preservation include agonal state, post-mortem delay, temperature of storage and procedures of tissue preservation. Examination of DNA and RNA preservation is best done by using bioanalyzer technologies instead of less sensitive methods such as agarose gels. Adequate RNA preservation is mandatory in RNA microarray studies and adequate controls are necessary for proper PCR validation. Like for RNA, the preservation of proteins is not homogeneous since some molecules are more vulnerable than others. This aspect is crucial in the study of proteins including expression levels and possible post-translational modifications. Similarly, the reliability of functional and enzymatic studies in human post-mortem brain largely depends on protein preservation. Much less is known about other aspects, such as the effects of putative deleterious factors on epigenetic events such as methylation of CpGs in gene promoters, nucleosome preservation, histone modifications, and conservation of microRNA species. Most brains are appropriate for morphological approaches but not all brains are useful for certain biochemical and molecular studies.
引用
收藏
页码:181 / 194
页数:14
相关论文
共 114 条
  • [21] Assessing quantitative post-mortem changes in the gray matter of the human frontal cortex proteome by 2-D DIGE
    Crecelius, Anna
    Goetz, Andrea
    Arzberger, Thomas
    Froehlich, Thomas
    Arnold, Georg J.
    Ferrer, Isidro
    Kretzschmar, Hans A.
    [J]. PROTEOMICS, 2008, 8 (06) : 1276 - 1291
  • [22] Recovery and expression of messenger RNA from postmortem human brain tissue
    Cummings, TJ
    Strum, JC
    Yoon, LW
    Szymanski, MH
    Hulette, CM
    [J]. MODERN PATHOLOGY, 2001, 14 (11) : 1157 - 1161
  • [23] Abnormal group I metabotropic glutamate receptor expression and signaling in the frontal cortex in Pick disease
    Dalfó, E
    Albasanz, JL
    Rodríguez, A
    Martín, M
    Ferrer, I
    [J]. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 2005, 64 (07) : 638 - 647
  • [24] Dalfó E, 2004, BRAIN PATHOL, V14, P388
  • [25] Abnormal α-synuclein interactions with rab3a and rabphilin in diffuse Lewy body disease
    Dalfó, E
    Barrachina, M
    Rosa, JL
    Ambrosio, S
    Ferrer, I
    [J]. NEUROBIOLOGY OF DISEASE, 2004, 16 (01) : 92 - 97
  • [26] α-Synuclein binding to rab3a in multiple system atrophy
    Dalfó, E
    Ferrer, I
    [J]. NEUROSCIENCE LETTERS, 2005, 380 (1-2) : 170 - 175
  • [27] Abnormal α-synuclein interactions with rab proteins in α-synuclein A30P transgenic mice
    Dalfó, E
    Gómez-Isla, T
    Rosa, JL
    Bodelón, MN
    Tejedor, MC
    Barrachina, M
    Ambrosio, S
    Ferrer, I
    [J]. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 2004, 63 (04) : 302 - 313
  • [28] Daniel S E, 1993, J Neural Transm Suppl, V39, P165
  • [29] Díaz-Hernández M, 2003, J NEUROSCI, V23, P11653
  • [30] Postmortem delay has minimal effect on brain RNA integrity
    Ervin, John F.
    Heinzen, Erin L.
    Cronin, Kenneth D.
    Goldstein, David
    Szymanski, Mari H.
    Burke, James R.
    Welsh-Bohmer, Kathleen A.
    Hulette, Christine M.
    [J]. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 2007, 66 (12) : 1093 - 1099