Determination of the docetaxel vehicle, polysorbate 80, in patient samples by liquid chromatography-tandem mass spectrometry

被引:37
作者
Sparreboom, A
Zhao, M
Brahmer, JR
Verweij, J
Baker, SD
机构
[1] Sidney Kimmel Comprehens Canc Ctr Johns Hopkins, Div Expt Therapeut, Baltimore, MD 21231 USA
[2] Rotterdam Canc Inst, Lab Expt Chemotherapy & Pharmacol, Dept Med Oncol, Daniel den Hoed Klin, NL-3075 EA Rotterdam, Netherlands
[3] Univ Rotterdam Hosp, NL-3075 EA Rotterdam, Netherlands
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 773卷 / 02期
关键词
docetaxel; polysorbate; 80;
D O I
10.1016/S1570-0232(02)00167-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new simple method was developed for the quantitative determination of the docetaxel (Taxotere) vehicle, polysorbate 80 (Tween 80), in human plasma. Calibration curves were constructed in the range of 1-100 mug/ml, using paclitaxel (0.01 mM) as internal standard, and were analyzed using a power fit with equal weighting. Sample pretreatment involved a one-step extraction with acetonitrile-n-butyl chloride (1:4, v/v). The analytes were separated on a Waters X-Terra MS column (50x2.1 mm I.D.) packed with 3.5-mum ODS material, and eluted with methanol-water (9:1, v/v) containing 0.1% formic acid. The column effluent was monitored by tandem mass spectrometry with electrospray ionization. The overall extraction efficiency was 50-60%, with values for precision and accuracy of less than or equal to16% and <15% relative error, respectively. Our current method is similar to60-100-fold more sensitive than previous assays, and will be used to define Tween 80 disposition in patients receiving Taxotere. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:183 / 190
页数:8
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