The effect of supplementary UV-B treatment on the essential oils of glasshouse-grown sweet basil (Ocimum basilicum L.) was examined. Two weeks treatment with supplementary W-B given in the early morning was found to enhance the levels of most of the major volatiles, both phenyl-propanoids (eugenol, methyl eugenol) and terpenoids, notably linalool, 1,8-cineole and trans-beta-ocimene. The phenyl-propanoids were sensitive to UV-B at an earlier developmental stage than the terpenoids. Overall, the effect of UV-B was a nearly fourfold stimulation in the oldest plants examined. (C) 1999 Elsevier Science Ltd. All rights reserved.