Inhibition of replication of rinderpest virus by 5-fluorouracil

被引：14

作者：

Ghosh, A ^{[1
]}

Nayak, R ^{[1
]}

Shaila, MS ^{[1
]}

机构：

[1] INDIAN INST SCI,DEPT MICROBIOL & CELL BIOL,BANGALORE 560012,KARNATAKA,INDIA

来源：

ANTIVIRAL RESEARCH | 1996年 / 31卷 / 1-2期

关键词：

rinderpest virus; 5-fluorouracil; virus transcription; antiviral drug;

D O I：

10.1016/0166-3542(96)00943-6

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

5-Fluorouracil (5FU), an analogue of uracil, was found to inhibit the production of infectious particles of rinderpest virus (RPV) in Vero cells (African green monkey kidney cells) by 99%; at a concentration of 1 mu g/ml. The levels of individual mRNA specific for five of the virus genes were also reduced drastically, while the level of mRNA for a cellular housekeeping gene-glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-was unaltered by fluorouracil treatment of infected cells. Both virus RNA and protein synthesis showed inhibition in a dose-dependent manner. The virions which budded out of 5-fluorouracil-treated cells also contained reduced amounts of virus proteins compared with virus particles from untreated cells.

引用

页码：35 / 44

页数：10

共 48 条

[1] A POINT MUTATION IN THE POLIOVIRUS POLYMERASE GENE DETERMINES A COMPLEMENTABLE TEMPERATURE-SENSITIVE DEFECT OF RNA REPLICATION [J].