Molecular characterization of human Argonaute-containing ribonucleoprotein complexes and their bound target mRNAs

被引：277

作者：

Landthaler, Markus ^{[2
]}

Gaidatzis, Dimos ^{[1
]}

Rothballer, Andrea ^{[2
]}

Chen, Po Yu ^{[2
]}

Soll, Steven Joseph ^{[2
]}

Dinic, Lana ^{[2
]}

Ojo, Tolulope ^{[2
]}

Hafner, Markus ^{[2
]}

Zavolan, Mihaela ^{[1
]}

Tuschl, Thomas ^{[2
]}

机构：

[1] Univ Basel, Biozentrum, Swiss Inst Bioinformat, CH-4056 Basel, Switzerland

[2] Rockefeller Univ, Howard Hughes Med Inst, Lab RNA Mol Biol, New York, NY 10065 USA

来源：

RNA | 2008年 / 14卷 / 12期

关键词：

microRNA; Argonaute; GW182/TNRC6; microRNA targets; immunoprecipitation;

D O I：

10.1261/rna.1351608

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

microRNAs (miRNAs) regulate the expression of mRNAs in animals and plants through miRNA-containing ribonucleoprotein particles (RNPs). At the core of these miRNA silencing effector complexes are the Argonaute (AGO) proteins that bind miRNAs and mediate target mRNA recognition. We generated HEK293 cell lines stably expressing epitope-tagged human AGO proteins and other RNA silencing-related proteins and used these cells to purify miRNA-containing RNPs. Mass spectrometric analyses of the proteins associated with different AGO proteins revealed a common set of helicases and mRNA-binding proteins, among them the three trinucleotide repeat containing proteins 6 (TNRC6A,-B,-C). mRNA microarray analyses of these miRNAassociated RNPs revealed that AGO and TNRC6 proteins bind highly similar sets of transcripts enriched in binding sites for highly expressed endogenous miRNAs, indicating that the TNRC6 proteins are a component of the mRNA-targeting miRNA silencing complex. Together with the very similar proteomic composition of each AGO complex, this result suggests substantial functional redundancy within families of human AGO and TNRC6 proteins. Our results further demonstrate that we have developed an effective biochemical approach to identify physiologically relevant human miRNA targets.

引用

页码：2580 / 2596

页数：17

共 104 条

[1] The functions of animal microRNAs [J].

Ambros, V .

NATURE, 2004, 431 (7006) :350-355

[2] The impact of microRNAs on protein output [J].

Baek, Daehyun ;

Villen, Judit ;

Shin, Chanseok ;

Camargo, Fernando D. ;

Gygi, Steven P. ;

Bartel, David P. .

NATURE, 2008, 455 (7209) :64-U38

[3] Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation [J].

Bagga, S ;

Bracht, J ;

Hunter, S ;

Massirer, K ;

Holtz, J ;

Eachus, R ;

Pasquinelli, AE .

CELL, 2005, 122 (04) :553-563

[4] Localization of the developmental timing regulator Lin28 to mRNP complexes, P-bodies and stress granules [J].

Balzer, Erica ;

Moss, Eric G. .

RNA BIOLOGY, 2007, 4 (01) :16-25

[5] Epstein-Barr virus-encoded microRNA miR-BART2 down-regulates the viral DNA polymerase BALF5 [J].

Barth, Stephanie ;

Pfuhl, Thorsten ;

Mamiani, Alfredo ;

Ehses, Claudia ;

Roemer, Klaus ;

Kremmer, Elisabeth ;

Jaeker, Christoph ;

Hoeck, Julia ;

Meister, Gunter ;

Graesser, Friedrich A. .

NUCLEIC ACIDS RESEARCH, 2008, 36 (02) :666-675

[6] MRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes [J].

Behm-Ansmant, Isabelle ;

Rehwinkel, Jan ;

Doerks, Tobias ;

Stark, Alexander ;

Bork, Peer ;

Izaurralde, Elisa .

GENES & DEVELOPMENT, 2006, 20 (14) :1885-1898

[7] Identification of human microRNA targets from isolated argonaute protein complexes [J].

Beitzinger, Michaela ;

Peters, Lasse ;

Zhu, Jia Yun ;

Kremmer, Elisabeth ;

Meister, Gunter .

RNA BIOLOGY, 2007, 4 (02) :76-84

[8] Role for a bidentate ribonuclease in the initiation step of RNA interference [J].

Bernstein, E ;

Caudy, AA ;

Hammond, SM ;

Hannon, GJ .

NATURE, 2001, 409 (6818) :363-366

[9] Relief of microRNA-mediated translational repression in human cells subjected to stress [J].

Bhattacharyya, Suvendra N. ;

Habermacher, Regula ;

Martine, Ursula ;

Closs, Ellen I. ;

Filipowicz, Witold .

CELL, 2006, 125 (06) :1111-1124

[10] MicroRNA functions [J].

Bushati, Natascha ;

Cohen, Stephen M. .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2007, 23 :175-205

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