The bacterial magnesium transporter CorA can functionally substitute for its putative homologue Mrs2p in the yeast inner mitochondrial membrane

被引:145
作者
Bui, DM [1 ]
Gregan, J [1 ]
Jarosch, E [1 ]
Ragnini, A [1 ]
Schweyen, RJ [1 ]
机构
[1] Univ Vienna, Inst Microbiol & Genet, Dept Genet & Microbiol, Vienna Bioctr, A-1030 Vienna, Austria
关键词
D O I
10.1074/jbc.274.29.20438
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The yeast nuclear gene MRS2 encodes a protein of 54 kDa, the presence of which has been shown to be essential for the splicing of group II intron RNA in mitochondria and, independently, for the maintenance of a functional respiratory system. Here we show that the MRS2 gene product (Mrs2p) is an integral protein of the inner mitochondrial membrane. It appears to be inserted into this membrane by virtue of two neighboring membrane spanning domains in its carboxyl-terminal half. A large amino-terminal and a shorter carboxyl-terminal part are likely to be exposed to the matrix space. Structural features and a short sequence motif indicate that Mrs2p may be related to the bacterial CorA Mg2+ transporter. In fact, overexpression of the CorA gene in yeast partially suppresses the pet(-) phenotype of an mrs2 disrupted yeast strain. Disruption of the MRS2 gene leads to a significant decrease in total magnesium content of mitochondria which is compensated for by the overexpression of the CorA gene. Mutants lacking or overproducing Mrs2p exhibit phenotypes consistent with the involvement of Mrs2p in mitochondrial Mg2+ homeostasis.
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页码:20438 / 20443
页数:6
相关论文
共 28 条
[1]   Regulation of cellular Mg2+ by Saccharomyces cerevisiae [J].
Beeler, T ;
Bruce, K ;
Dunn, T .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1997, 1323 (02) :310-318
[2]  
DAUM G, 1982, J BIOL CHEM, V257, P3028
[3]   Ataxia, arrhythmia and ion-channel gene defects [J].
Doyle, JL ;
Stubbs, L .
TRENDS IN GENETICS, 1998, 14 (03) :92-98
[4]   The molecular biology of metal ion transport in Saccharomyces cerevisiae [J].
Eide, DJ .
ANNUAL REVIEW OF NUTRITION, 1998, 18 :441-469
[5]   MECHANISMS OF MAGNESIUM TRANSPORT [J].
FLATMAN, PW .
ANNUAL REVIEW OF PHYSIOLOGY, 1991, 53 :259-271
[6]   ASSOCIATION OF DEGRADATION AND SECRETION OF 3 CHIMERIC POLYPEPTIDES IN ESCHERICHIA-COLI [J].
GENTZ, R ;
KUYS, Y ;
ZWIEB, C ;
TAATJES, D ;
TAATJES, H ;
BANNWARTH, W ;
STUEBER, D ;
IBRAHIMI, I .
JOURNAL OF BACTERIOLOGY, 1988, 170 (05) :2212-2220
[7]   NEW YEAST-ESCHERICHIA-COLI SHUTTLE VECTORS CONSTRUCTED WITH INVITRO MUTAGENIZED YEAST GENES LACKING 6-BASE PAIR RESTRICTION SITES [J].
GIETZ, RD ;
SUGINO, A .
GENE, 1988, 74 (02) :527-534
[8]   YEAST ESCHERICHIA-COLI SHUTTLE VECTORS WITH MULTIPLE UNIQUE RESTRICTION SITES [J].
HILL, JE ;
MYERS, AM ;
KOERNER, TJ ;
TZAGOLOFF, A .
YEAST, 1986, 2 (03) :163-167
[9]   Mrs5p, an essential protein of the mitochondrial intermembrane space, affects protein import into yeast mitochondria [J].
Jarosch, E ;
Tuller, G ;
Daum, G ;
Waldherr, M ;
Voskova, A ;
Schweyen, RJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (29) :17219-17225
[10]   ISOTACHOPHORETIC SEPARATION OF ALKALI AND ALKALINE-EARTH METAL-CATIONS IN WATER-POLYETHYLENE GLYCOL MIXTURES [J].
KANIANSKY, D ;
ZELENSKY, I ;
VALASKOVA, I ;
MARAK, J ;
ZELENSKA, V .
JOURNAL OF CHROMATOGRAPHY, 1990, 502 (01) :143-153