RNA editing site recognition in heterologous plant mitochondria

被引:15
作者
Choury, David [1 ]
Araya, Alejandro [1 ]
机构
[1] CNRS, UMR 5097, Lab Replicat & Express Genomes Eucaryotes & Retro, F-33076 Bordeaux, France
关键词
RNA editing; electroporation; mitochondria; in organello; cox2; atp9;
D O I
10.1007/s00294-006-0100-3
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
RNA editing is a process that modifies the information content of mitochondrial messenger RNAs in flowering plants changing specific cytosine residues into uridine. To gain insight into editing site recognition, we used electroporation to introduce engineered wheat (Triticum aestivum) or potato (Solanum tuberosum) mitochondrial cox2 genes, and an atp9-containing chimeric gene, into non-cognate mitochondria, and observed the efficiency of editing in these contexts. Both wheat and potato mitochondria were able to express "foreign" constructs, and their products were properly spliced. Seventeen and twelve editing sites are present in the coding regions of wheat and potato cox2 transcripts, respectively. Eight are common to both plants, whereas nine are specific to wheat, and four to potato. An analogous situation is found for the atp9 mRNA coding regions from these species. We found that both mitochondria were able to recognize sites that are already present as T at the genomic level, making RNA editing unnecessary for that specific residue in the cognate organelle. Our results demonstrate that non-cognate mitochondria are able to edit residues that are not edited in their own transcripts, and support the hypothesis that the same trans-acting factor may recognize several editing sites.
引用
收藏
页码:405 / 416
页数:12
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