Silicon photonic wire biosensor array for multiplexed real-time and label-free molecular detection

被引：152

作者：

Densmore, A. ^{[1
]}

Vachon, M. ^{[1
]}

Xu, D. -X. ^{[1
]}

Janz, S. ^{[1
]}

Ma, R. ^{[1
]}

Li, Y. -H. ^{[2
]}

Lopinski, G. ^{[2
]}

Delage, A. ^{[1
]}

Lapointe, J. ^{[1
]}

Luebbert, C. C. ^{[3
]}

Liu, Q. Y. ^{[3
]}

Cheben, P. ^{[1
]}

Schmid, J. H. ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Microstruct Sci, Ottawa, ON K1A 0R6, Canada

[2] Natl Res Council Canada, Steacie Inst Mol Sci, Ottawa, ON K1A 0R6, Canada

[3] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

来源：

OPTICS LETTERS | 2009年 / 34卷 / 23期

关键词：

HIGH-SENSITIVITY; RESONATOR; SENSORS;

D O I：

10.1364/OL.34.003598

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We demonstrate a silicon photonic wire waveguide biosensor array chip for the simultaneous monitoring of different molecular binding reactions. The chip is compatible with automated commercial spotting tools and contains a monolithically integrated microfluidic channel for sample delivery. Each array sensor element is a 1.8-mm-long spiral waveguide folded within a 130 mu m diameter spot and is incorporated in a balanced Mach-Zehnder interferometer with a near temperature independent response. The sensors are arranged in a 400 mu m spacing grid pattern and are addressed through cascaded 1 x 2 optical power splitters using light from a single input fiber. We demonstrate the real-time monitoring of antibody-antigen reactions using complementary and mismatched immunoglobulin G receptor-analyte pairs and bovine serum albumin. The measured level of detection for each sensor element corresponds to a surface coverage of less than 0.3 pg/mm(2). (C) 2009 Optical Society of America

引用

页码：3598 / 3600

页数：3

共 12 条

[1] Label-free, single-molecule detection with optical microcavities
Armani, Andrea M.
Kulkarni, Rajan P.
Fraser, Scott E.
Flagan, Richard C.
Vahala, Kerry J.
[J]. SCIENCE, 2007, 317 (5839) : 783 - 787
[2] Sensitive disk resonator photonic biosensor
Boyd, RW
Heebner, JE
[J]. APPLIED OPTICS, 2001, 40 (31) : 5742 - 5747
[3] Silicon-on-Insulator microring resonator for sensitive and label-free biosensing
De Vos, Katrien
Bartolozzi, Irene
Schacht, Etienne
Bienstman, Peter
Baets, Roel
[J]. OPTICS EXPRESS, 2007, 15 (12): : 7610 - 7615
[4] Spiral-path high-sensitivity silicon photonic wire molecular sensor with temperature-independent response
Densmore, A.
Xu, D. -X.
Janz, S.
Waldron, P.
Mischki, T.
Lopinski, G.
Delage, A.
Lapointe, J.
Cheben, P.
Lamontagne, B.
Schmid, J. H.
[J]. OPTICS LETTERS, 2008, 33 (06) : 596 - 598
[5] A silicon-on-insulator photonic wire based evanescent field sensor
Densmore, A.
Xu, D. X.
Waldron, P.
Janz, S.
Cheben, P.
Lapointe, J.
Delage, A.
Lamontagne, B.
Schmid, J. H.
Post, E.
[J]. IEEE PHOTONICS TECHNOLOGY LETTERS, 2006, 18 (21-24) : 2520 - 2522
[6] Protein absorption and ellipsometry in biomaterial research
Elwing, H
[J]. BIOMATERIALS, 1998, 19 (4-5) : 397 - 406
[7] Integrated optics ring-resonator sensors for protein detection
Ksendzov, A
Lin, Y
[J]. OPTICS LETTERS, 2005, 30 (24) : 3344 - 3346
[8] Integrated optical Mach-Zehnder biosensor
Luff, BJ
Wilkinson, JS
Piehler, J
Hollenbach, U
Ingenhoff, J
Fabricius, N
[J]. JOURNAL OF LIGHTWAVE TECHNOLOGY, 1998, 16 (04) : 583 - 592
[9] PRINCIPLES AND SENSITIVITIES OF INTEGRATED OPTICAL AND SURFACE-PLASMON SENSORS FOR DIRECT AFFINITY SENSING AND IMMUNOSENSING
LUKOSZ, W
[J]. BIOSENSORS & BIOELECTRONICS, 1991, 6 (03) : 215 - 225
[10] Optical biosensor microsystems based on the integration of highly sensitive Mach-Zehnder interferometer devices
Sepulveda, B.
Sanchez del Rio, J.
Moreno, M.
Blanco, F. J.
Mayora, K.
Dominguez, C.
Lechuga, L. M.
[J]. JOURNAL OF OPTICS A-PURE AND APPLIED OPTICS, 2006, 8 (07): : S561 - S566

← 1 2 →