Synthesis of conjugates for a barbiturate screening assay

被引：6

作者：

Adamczyk, M

Grote, J

Douglas, J

Dubler, R

Harrington, C

机构：

[1] Department of Chemistry (D9NM), Abbott Diagnostics Division, Abbott Laboratories, Abbott Park, IL 60064-3500

来源：

BIOCONJUGATE CHEMISTRY | 1997年 / 8卷 / 03期

关键词：

D O I：

10.1021/bc9700345

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel derivatives of barbiturates functionalized with free carboxylic acids were designed and synthesized. Coupling of 5-cyclopentyl-5-carboxycrotylbarbituric acid via its active ester to an aminofluorescein derivative produced a fluorescent tracer. Conjugation of the 5-cyclopentenyl-5-carboxyethylbarbituric acid via its mixed anhydride to thyroglobulin allowed for subsequent development of a polyclonal antibody which was evaluated for binding in a fluorescence polarization immunoassay format with various barbiturates. The binding studies showed good cross-reactivity of a variety of barbiturates containing both aromatic and aliphatic 5-substituents with the tested antisera. The relationship between the immunogen architecture, the chemical structure of the binding analytes, and the characteristics of the antisera is also presented.

引用

页码：281 / 288

页数：8

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