Synthesis of Fe3O4@SiO2@PMMA Core-Shell-Shell Magnetic Microspheres for Highly Efficient Enrichment of Peptides and Proteins for MALDI-ToF MS Analysis

被引：341

作者：

Chen, Hemei ^{[1
,2
]}

Deng, Chunhui ^{[1
,2
]}

Zhang, Xiangmin ^{[1
,2
]}

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

[2] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 03期

基金：

中国国家自然科学基金;

关键词：

magnetic properties; microspheres; peptides; polymers; sol-gel processes; IONIZATION MASS-SPECTROMETRY; SILICA MICROSPHERES; SEMICONDUCTOR NANOCRYSTALS; RAPID ENRICHMENT; FACILE SYNTHESIS; PHOSPHOPEPTIDES; PROTEOLYSIS; MATRIX; IMMOBILIZATION; NANOPARTICLES;

D O I：

10.1002/anie.200904885

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Particularly enriching: Core-shell-shell Fe 3O4@SiO2@PMMA composite microspheres (see picture; PMMA = poly(methyl methacrylate)) were synthesized readily by combining a sol-gel approach with a seeded aqueous-phase radical polymerization method. The microspheres were successfully applied toward the effective, rapid, and convenient enrichment of low-concentration peptides and proteins for mass spectrometric analysis. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：607 / 611

页数：5

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