A Facile Synthesis Approach to C8-Functionalized Magnetic Carbonaceous Polysaccharide Microspheres for the Highly Efficient and Rapid Enrichment of Peptides and Direct MALDI-TOF-MS Analysis

被引：66

作者：

Chen, Hemei

Deng, Chunhui ^{[1
]}

Li, Yan

Dai, Ying

Yang, Penguan

Zhang, Xiangmin

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

来源：

ADVANCED MATERIALS | 2009年 / 21卷 / 21期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

IONIZATION MASS-SPECTROMETRY; CORE-SHELL MICROSPHERES; LOW-ABUNDANCE PEPTIDES; SILICA MICROSPHERES; LIQUID-CHROMATOGRAPHY; MATRIX; NANOPARTICLES; NANOTUBES; PROTEIN; PHOSPHOPEPTIDES;

D O I：

10.1002/adma.200802260

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Biocompatible C-8-functionalized magnetic carbonaceous polysaccharide microspheres are synthesized via a facile, low-cost, and large-scale route, and their use for the enrichment of peptides from protein digest mixtures is presented. The process of enrichment is very simple, quick, and efficient. Peptides loaded onto the C-8-functionalized magnetic carbonaceous polysaccharide microspheres can be directly analyzed by MALDI-TOF-MS without prior elution from the microspheres.

引用

页码：2200 / +

页数：7

共 48 条

[1] And now for the proteome ... [J].