Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A

被引：54

作者：

Clow, Fiona ^{[1
,2
]}

Fraser, John D. ^{[1
,2
]}

Proft, Thomas ^{[1
,2
]}

机构：

[1] Univ Auckland, Dept Mol Med & Pathol, Sch Med Sci, Auckland 1, New Zealand

[2] Univ Auckland, Maurice Wilkins Ctr Mol Biodiscovery, Auckland 1, New Zealand

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 09期

关键词：

biacore; biosensor; fibronectin-binding protein; human factor H; plasmon surface resonance; protein immobilization; sortase;

D O I：

10.1007/s10529-008-9718-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The immobilization of proteins to surfaces is an active area of research due to strong interest in protein-based sensors. Here, we describe a novel method for immobilizing ligand proteins onto Biacore sensor chips using the transpeptidase activity of Staphylococcus aureus sortase A (SrtA). This method provides a robust and gentle approach for the site-directed, covalent coupling of proteins to biosensor chips. Notably, the high specificity of the sortase allows immobilization of proteins from less than pure protein samples allowing short cuts in protein purification protocols.

引用

页码：1603 / 1607

页数：5

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