In vitro assay of Staphylococcus aureus enterotoxin a activity in food

被引:14
作者
Rasooly, L
Rose, NR
Shah, DB
Rasooly, A
机构
[1] US FDA, DIV MICROBIOL STUDIES, WASHINGTON, DC 20204 USA
[2] JOHNS HOPKINS UNIV, DEPT MOL MICROBIOL & IMMUNOL, BALTIMORE, MD USA
[3] JOHNS HOPKINS UNIV, SCH MED, DEPT PATHOL, BALTIMORE, MD 21205 USA
关键词
D O I
10.1128/AEM.63.6.2361-2365.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Staphylococcus aureus enterotoxin A (SEA) is a leading cause of food poisoning. The current test for functional activity of SEA requires monkeys or kittens. The major drawbacks of animal assays are lack of quantitation, poor reproducibility, low sensitivity and high cost. In this report we describe and evaluate an alternative assay using T-cell proliferation to measure SEA activity in food. Human and rat lymphocytes proliferate in response to concentrations of SEA as low as 1 pg/ml, well below the pathogenic dose of 100 ng. This proliferation assay is highly sensitive, quantitative, and simple. Nonradioactive assays of T-cell proliferation were also suitable for detecting and measuring SEA, although with a 10-fold lower sensitivity. To evaluate the utility of this assay for food testing, four different food samples were mixed with SEA. In each sample, SEA was detected at a concentration of 1 ng/ml. Heat-inactivated SEA produced no detectable proliferation. These results demonstrate that an in vitro cell proliferation assay is an advantageous alternative to existing animal assays for measuring SEA activity in food.
引用
收藏
页码:2361 / 2365
页数:5
相关论文
共 12 条
[1]  
ARCHER D L, 1988, Clinical Microbiology Reviews, V1, P377
[2]  
BERGDOLL MS, 1988, METHOD ENZYMOL, V165, P324
[3]  
BERGDOLL MS, 1972, STAPHYLOCOCCI, P301
[4]  
COLIGAN JE, 1990, CURRENT PROTOCOLS IM
[5]   ESTIMATION OF HUMAN DOSE OF STAPHYLOCOCCAL ENTEROTOXIN-A FROM A LARGE OUTBREAK OF STAPHYLOCOCCAL FOOD POISONING INVOLVING CHOCOLATE MILK [J].
EVENSON, ML ;
HINDS, MW ;
BERNSTEIN, RS ;
BERGDOLL, MS .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1988, 7 (04) :311-316
[6]  
FRIEDMAN SM, 1993, RHEUM DIS CLIN N AM, V19, P207
[7]   LACK OF COMPLETE CORRELATION BETWEEN EMETIC AND T-CELL-STIMULATORY ACTIVITIES OF STAPHYLOCOCCAL ENTEROTOXINS [J].
HARRIS, TO ;
GROSSMAN, D ;
KAPPLER, JW ;
MARRACK, P ;
RICH, RR ;
BETLEY, MJ .
INFECTION AND IMMUNITY, 1993, 61 (08) :3175-3183
[8]   SIMPLE SOLUTIONS TO FALSE-POSITIVE STAPHYLOCOCCAL-ENTEROTOXIN ASSAYS WITH SEAFOOD TESTED WITH AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY KIT (TECRA) [J].
PARK, CE ;
AKHTAR, M ;
RAYMAN, MK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (07) :2210-2213
[9]   EVALUATION OF A COMMERCIAL ENZYME-IMMUNOASSAY KIT (RIDASCREEN) FOR DETECTION OF STAPHYLOCOCCAL ENTEROTOXINS A, B, C, D, AND E IN FOODS [J].
PARK, CE ;
AKHTAR, M ;
RAYMAN, MK .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (02) :677-681
[10]   INACTIVATION OF STAPHYLOCOCCAL ENTEROTOXINS BY HEAT AND REACTIVATION BY HIGH PH TREATMENT [J].
SCHWABE, M ;
NOTERMANS, S ;
BOOT, R ;
TATINI, SR ;
KRAMER, J .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1990, 10 (01) :33-42