Competitive Incorporation of Homologous Gene Segments of Influenza A Virus into Virions

被引：30

作者：

Inagaki, Arisa ^{[1
]}

Goto, Hideo ^{[1
]}

Kakugawa, Satoshi ^{[1
]}

Ozawa, Makoto ^{[2
,3
]}

Kawaoka, Yoshihiro ^{[1
,4
,5
,6
]}

机构：

[1] Univ Tokyo, Inst Med Sci, Dept Microbiol & Immunol, Div Virol,Minato Ku, Shiroganedai, Tokyo, Japan

[2] Kagoshima Univ, Joint Fac Vet Med, Lab Anim Hyg, Kagoshima 890, Japan

[3] Kagoshima Univ, Joint Fac Vet Med, Transboundary Anim Dis Ctr, Kagoshima 890, Japan

[4] Univ Tokyo, Inst Med Sci, Int Res Ctr Infect Dis, Dept Special Pathogens,Minato Ku, Shiroganedai, Tokyo, Japan

[5] Univ Wisconsin, Sch Vet Med, Dept Pathobiol Sci, Madison, WI 53706 USA

[6] Japan Sci & Technol Agcy, ERATO Infect Induced Host Responses Project, Saitama, Japan

来源：

JOURNAL OF VIROLOGY | 2012年 / 86卷 / 18期

基金：

日本科学技术振兴机构;

关键词：

GENOMIC RNA SEGMENTS; PACKAGING SIGNALS; VIRAL-RNA; RIBONUCLEOPROTEIN COMPLEXES; REPLICATION; REGIONS; PB2; PA;

D O I：

10.1128/JVI.01204-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By using two reporter protein-encoding virus-like RNAs derived from identical viral RNA (vRNA) segments, we assessed their incorporation efficiency into single progeny virions. Most plaques formed by the recombinant viruses that were generated in cells positive for both reporter genes expressed only one or the other protein. These results suggest that two virus-like RNAs encoding different reporter proteins compete for incorporation into virions, and individual influenza virions incorporate single, but not multiple, copies of homologous vRNA segments.

引用

页码：10200 / 10202

页数：3

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