Differential regulation of the human and murine CD34 genes in hematopoietic stem cells

被引：60

作者：

Okuno, Y

Iwasaki, H

Huettner, CS

Radomska, HS

Gonzalez, DA

Tenen, DG

Akashi, K

机构：

[1] Harvard Univ, Inst Med, Div Hematol Oncol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Canc Immunol & AIDS, Boston, MA 02115 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2002年 / 99卷 / 09期

关键词：

D O I：

10.1073/pnas.092027799

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Human CD34 (hCD34)-positive cells are used currently as a source for hematopoietic transplantation in humans. However, in steady-state murine hematopoiesis, hematopoietic stem cells (HSCs) with long-term reconstitution activity are found almost exclusively in the murine CD34 (mCD34)-negative to low fraction. To evaluate the possible differences in hCD34 and mCD34 gene expression in hematopoiesis, we made transgenic mouse strains with human genomic Fill artificial chromosome clones spanning the entire hCD34 genomic locus. In all transgenic mouse strains, a vast majority of phenotypic and functional HSC populations including mCD34(-/lo) express the hCD34 transgene. These data strongly support the notion that hCD34(+) human bone marrow cells contain long-term HSCs that can maintain hematopoiesis throughout life.

引用

收藏

页码：6246 / 6251

页数：6

相关论文

共 29 条

[11] Transgenic analysis of a 100-kb human β-globin cluster-containing DNA fragment propagated as a bacterial artificial chromosome [J].

Kaufman, RM ;

Pham, CTN ;

Ley, TJ .

BLOOD, 1999, 94 (09) :3178-3184

[12] Identification of clonogenic common lymphoid progenitors in mouse bone marrow [J].

Kondo, M ;

Weissman, IL ;

Akashi, K .

CELL, 1997, 91 (05) :661-672

[13] Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell [J].

Krause, DS ;

Theise, ND ;

Collector, MI ;

Henegariu, O ;

Hwang, S ;

Gardner, R ;

Neutzel, S ;

Sharkis, SJ .

CELL, 2001, 105 (03) :369-377

[14] Purified hematopoietic stem cells can differentiate into hepatocytes in vivo [J].

Lagasse, E ;

Connors, H ;

Al-Dhalimy, M ;

Reitsma, M ;

Dohse, M ;

Osborne, L ;

Wang, X ;

Finegold, M ;

Weissman, IL ;

Grompe, M .

NATURE MEDICINE, 2000, 6 (11) :1229-1234

[15] Transplantation of allogeneic CD34(+) blood cells [J].

Link, H ;

Arseniev, L ;

Bahre, O ;

Kadar, JG ;

Diedrich, H ;

Poliwoda, H .

BLOOD, 1996, 87 (11) :4903-4909

[16] Transplantation with selected autologous peripheral blood CD34+Thy1+ hematopoietic stem cells (HSCs) in multiple myeloma:: Impact of HSC dose on engraftment, safety, and immune reconstitution [J].

Michallet, M ;

Philip, T ;

Philip, I ;

Godinot, H ;

Sebban, C ;

Salles, G ;

Thiebaut, A ;

Biron, P ;

Lopez, F ;

Mazars, P ;

Roubi, N ;

Leemhuis, T ;

Hanania, E ;

Reading, C ;

Fine, G ;

Atkinson, K ;

Juttner, C ;

Coiffier, B ;

Fière, D ;

Archimbaud, E .

EXPERIMENTAL HEMATOLOGY, 2000, 28 (07) :858-870

[17] THE LONG-TERM REPOPULATING SUBSET OF HEMATOPOIETIC STEM-CELLS IS DETERMINISTIC AND ISOLATABLE BY PHENOTYPE [J].

MORRISON, SJ ;

WEISSMAN, IL .

IMMUNITY, 1994, 1 (08) :661-673

[18] EXPRESSION AND FUNCTION OF C-KIT IN HEMATOPOIETIC PROGENITOR CELLS [J].

OGAWA, M ;

MATSUZAKI, Y ;

NISHIKAWA, S ;

HAYASHI, SI ;

KUNISADA, T ;

SUDO, T ;

KINA, T ;

NAKAUCHI, H ;

NISHIKAWA, SI .

JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 174 (01) :63-71

[19] Bone marrow cells regenerate infarcted myocardium [J].

Orlic, D ;

Kajstura, J ;

Chimenti, S ;

Jakoniuk, I ;

Anderson, SM ;

Li, BS ;

Pickel, J ;

McKay, R ;

Nadal-Ginard, B ;

Bodine, DM ;

Leri, A ;

Anversa, P .

NATURE, 2001, 410 (6829) :701-705

[20] Functional characterization of a novel hematopoietic stem cell and its place in the c-kit maturation pathway in bone marrow cell development [J].

Ortiz, M ;

Wine, JW ;

Lohrey, N ;

Ruscetti, FW ;

Spence, SE ;

Keller, JR .

IMMUNITY, 1999, 10 (02) :173-182