Allelopathic potential of Artemisia arborescens: Isolation, identification and quantification of phytotoxic compounds through fractionation-guided bioassays

被引:29
作者
Araniti, Fabrizio [1 ]
Lupini, Antonio [1 ]
Sorgona, Agostino [1 ]
Conforti, Filomena [2 ]
Marrelli, Mariangela [2 ]
Statti, Giancarlo Antonio [2 ]
Menichini, Francesco [2 ]
Abenavoli, Maria Rosa [1 ]
机构
[1] Univ Mediterranea Reggio Calabria, Dipartimento Biotecnol Monitoraggio Agroalimentar, Fac Agr, I-89124 Calabria, RC, Italy
[2] Univ Calabria, Dipartimento Sci Farmaceut, Fac Farm & Sci Nutr & Salute, I-87030 Arcavacata Di Rende, CS, Italy
关键词
allelochemicals; allelopathy; dose-response curve; pulegone; root growth; seed germination; terpene; ALLELOCHEMICALS; GERMINATION; TARGET;
D O I
10.1080/14786419.2012.691491
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The aerial part of Artemisia arborescens L. (Asteraceae) was extracted with water and methanol, and both extracts were fractionated using n-hexane, chloroform, ethyl acetate and n-butanol. The potential phytotoxicity of both crude extracts and their fractions were assayed invitro on seed germination and root growth of lettuce (Lactuca sativa L.), a sensitive species largely employed in the allelopathy studies. The inhibitory activities were analysed by dose-response curves and the ED50 were estimated. Crude extracts strongly inhibited both germination and root growth processes. The fraction-bioassay indicated the following hierarchy of phytotoxicity for both physiological processes: ethyl acetaten-hexane> chloroformn-butanol. On the n-hexane fraction, GC-MS analyses were carried out to characterise and quantify some of the potential allelochemicals. Twenty-one compounds were identified and three of them, camphor, trans-caryophyllene and pulegone were quantified.
引用
收藏
页码:880 / 887
页数:8
相关论文
共 25 条
[1]   Phytotoxicity of Vulpia residues:: II.: Separation, identification, and quantitation of allelochemicals from Vulpia myuros [J].
An, M ;
Haig, T ;
Pratley, JE .
JOURNAL OF CHEMICAL ECOLOGY, 2000, 26 (06) :1465-1476
[2]   Isolation of bioactive allelochemicals from sunflower (variety Suncross-42) through fractionation-guided bioassays [J].
Anjum, Tehmina ;
Bajwa, Rukhsana .
NATURAL PRODUCT RESEARCH, 2010, 24 (18) :1783-1788
[3]  
Araniti F, 2012, ALLELOPATHY J, V29, P107
[4]  
Belz Regina G., 2005, Nonlinearity in Biology Toxicology and Medicine, V3, P173, DOI 10.2201/nonlin.003.02.002
[5]   Microtubules are an intracellular target of the plant terpene citral [J].
Chaimovitsh, David ;
Abu-Abied, Mohamad ;
Belausov, Eduard ;
Rubin, Baruch ;
Dudai, Nativ ;
Sadot, Einat .
PLANT JOURNAL, 2010, 61 (03) :399-408
[6]   Do germination indices adequately reflect allelochemical effects on the germination process? [J].
Chiapusio, G ;
Sánchez-Moreiras, AM ;
Reigosa, MJ ;
Gonzalez, L ;
Pellissier, F .
JOURNAL OF CHEMICAL ECOLOGY, 1997, 23 (11) :2445-2453
[7]   Effects of alfalfa leaf extracts and phenolic allelochemicals on early seedling growth and root morphology of alfalfa and barnyard grass [J].
Chon, SU ;
Choi, SK ;
Jung, S ;
Jang, HG ;
Pyo, BS ;
Kim, SM .
CROP PROTECTION, 2002, 21 (10) :1077-1082
[8]  
Duke SO, 2000, WEED RES, V40, P99, DOI 10.1046/j.1365-3180.2000.00161.x
[9]  
Duke SO, 2002, WEED SCI, V50, P138, DOI 10.1614/0043-1745(2002)050[0138:IPCFNF]2.0.CO
[10]  
2