Ultrastructural immunolocalization of basic fibroblast growth factor to lipid bodies and secretory granules in human mast cells

被引:22
作者
Dvorak, AM
Morgan, ES
Weller, PF
机构
[1] Beth Israel Deaconess Med Ctr, Dept Pathol, Boston, MA 02215 USA
[2] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02215 USA
[3] Harvard Univ, Sch Med, Dept Med, Boston, MA 02215 USA
来源
HISTOCHEMICAL JOURNAL | 2001年 / 33卷 / 07期
关键词
D O I
10.1023/A:1013771827069
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Isolated human lung mast cells were used to identify subcellular sites of basic fibroblast growth factor using a postembedding immunogold method. The factor was present in quantity in secretory granules and cytoplasmic lipid bodies. Cisterns of smooth endoplasmic reticulum and ribosome clusters, closely associated with lipid bodies, contained the factor as did the nuclear matrix. Factor-positive lipid bodies were adjacent to nuclear pores and often indented perinuclear cisternae. Altered secretory granules with reduced density, characteristic of secretion by piecemeal degranulation in mast cells, showed reduced gold label for basic fibroblast growth factor; small, electron-lucent (80-100 nm) transport vesicles near altered granules were labelled for the factor. Since these mature mast cells do not display extensive arrays of classical secretory organelles, such as rough endoplasmic reticulum. and Golgi structures, these new subcellular localizations for basic fibroblast growth factor suggest several possible alternative release routes for a cytokine devoid of a signal sequence characteristic of regulated secretory proteins.
引用
收藏
页码:397 / 402
页数:6
相关论文
共 41 条
[31]  
OKAYAMA Y, 1995, CHEM IMMUNOL, V61, P114
[32]  
QU ZH, 1995, LAB INVEST, V73, P339
[33]  
QU ZH, 1995, AM J PATHOL, V147, P564
[34]   Ultrastructural immunolocalization of basic fibroblast growth factor in mast cell secretory granules: Morphological evidence for bFGF release through degranulation [J].
Qu, ZH ;
Kayton, RJ ;
Ahmadi, P ;
Liebler, JM ;
Powers, MR ;
Planck, SR ;
Rosenbaum, JT .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1998, 46 (10) :1119-1128
[35]   Synthesis of basic fibroblast growth factor by murine mast cells - Regulation by transforming growth factor beta, tumor necrosis factor alpha, and stem cell factor [J].
Qu, ZH ;
Huang, XN ;
Ahmadi, P ;
Stenberg, P ;
Liebler, JM ;
Le, AC ;
Planck, SR ;
Rosenbaum, JT .
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1998, 115 (01) :47-54
[36]   THE NH2-TERMINAL EXTENSION OF HIGH-MOLECULAR-WEIGHT BFGF IS A NUCLEAR TARGETING SIGNAL [J].
QUARTO, N ;
FINGER, FP ;
RIFKIN, DB .
JOURNAL OF CELLULAR PHYSIOLOGY, 1991, 147 (02) :311-318
[37]  
REED JA, 1995, LAB INVEST, V72, P215
[38]   NUCLEAR AND CYTOPLASMIC LOCALIZATION OF DIFFERENT BASIC FIBROBLAST GROWTH-FACTOR SPECIES [J].
RENKO, M ;
QUARTO, N ;
MORIMOTO, T ;
RIFKIN, DB .
JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 144 (01) :108-114
[39]  
SCHULMAN ES, 1982, J IMMUNOL, V129, P2662
[40]   CAPILLARY ENDOTHELIAL-CELLS EXPRESS BASIC FIBROBLAST GROWTH-FACTOR, A MITOGEN THAT PROMOTES THEIR OWN GROWTH [J].
SCHWEIGERER, L ;
NEUFELD, G ;
FRIEDMAN, J ;
ABRAHAM, JA ;
FIDDES, JC ;
GOSPODAROWICZ, D .
NATURE, 1987, 325 (6101) :257-259