The protective effects of Achyranthes bidentata polypeptides against NMDA-induced cell apoptosis in cultured hippocampal neurons through differential modulation of NR2A- and NR2B-containing NMDA receptors

被引：95

作者：

Shen, Hongmei ^{[1
,2
]}

Yuan, Ying ^{[1
]}

Ding, Fei ^{[1
]}

Liu, Jie ^{[1
]}

Gu, Xiaosong ^{[1
]}

机构：

[1] Nantong Univ, Jiangsu Key Lab Neuroregenerat, Nantong 226001, Peoples R China

[2] Soochow Univ, Sch Med, Suzhou 215123, Peoples R China

来源：

BRAIN RESEARCH BULLETIN | 2008年 / 77卷 / 05期

关键词：

Achyranthes bidentata; N-methyl-D-aspartate (NMDA); Anti-apoptosis; NMDA receptors;

D O I：

10.1016/j.brainresbull.2008.08.002

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Achyranthes bidentata Blume is a commonly prescribed Chinese medicinal herb with a variety of pharmaceutical properties. From its aqueous extract we have separated important constituents, referred to as A. bidentata polypeptides (ABPP). In this study, the neuroprotective effect of ABPP against N-methyl-D-aspartate (NMDA)-induced cell apoptosis was investigated in cultured rat hippocampal neurons. The results of MTT assay, Hoechst/PI double staining and DNA ladder detection indicated that ABPP significantly attenuated, in a concentration-dependent manner, apoptotic cell damage induced by exposure of cultured hippocampal neurons to NMDA (100 mu M) for 30 min. The intracellular calcium measurement with fluo-3/AM revealed that ABPP antagonized the excess intracellular calcium triggered by NMDA. Furthermore, in addition to inhibiting the action of NR2B-containing NMDA receptors, ABPP can enhance the function of NR2A-containing NMDA receptors. Our data might suggest that ABPP may also prove to be a potential neuroprotective therapy owing to its differential modulation of NR2A- and NR2B-containing NMDA receptors. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：274 / 281

页数：8

共 42 条

[1]

Guidelines for the early management of patients with ischemic stroke - A scientific statement from the Stroke Council of the American Stroke Association [J].