Are ovine Leydig cells able to aromatize androgens?

被引:44
作者
Bilinska, B
Lesniak, M
Schmalz, B
机构
[1] Lab. Anim. Endocrinol. Tissue C., Institute of Zoology, Jagiellonian University, 30-060 Kraków
关键词
aromatase; inhibitor aromatase; immunohistochemistry; ram; in vitro;
D O I
10.1071/R96038
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The conversion of testosterone into oestradiol by ovine Leydig cells cultured in vitro was studied using the non-steroidal aromatase inhibitor CGS 16949A. Additionally, aromatase activity was detected by immunohistochemical staining of cultured Leydig cells or cryosections. The cells were obtained from testes of Polish Mountain rams 5-6 months old (immature) or 12-15 months old (mature). Leydig cells were cultured alone (controls) or incubated for 6 h in the presence of testosterone. Aromatase inhibitor was then added to the cultures which were incubated for a further 18 h. After a 24-h incubation period, testosterone and oestradiol secretion were determined by testing the culture medium using radioimmunological methods. The addition of testosterone to the culture medium enhanced oestradiol synthesis, suggesting that exogenous testosterone could also be aromatized to oestradiol by ovine Leydig cells in vitro. In the presence of CGS 16949A, the conversion of testosterone to oestradiol was significantly suppressed in a dose-dependent manner. All Leydig cells obtained from testes of mature rams and stained immunohistochemically were positive for aromatase, whereas Leydig cells from immature males were negative. The localization of immunoreactive aromatase appeared to be dependent on the age of the donor ram. It is suggested therefore, that mature Leydig cells in the ram are not only the site for testosterone synthesis, they are also capable of converting androgens into oestrogens.
引用
收藏
页码:193 / 199
页数:7
相关论文
共 43 条
[21]   IMMUNOCYTOCHEMICAL LOCALIZATION OF AROMATASE IN RAT TESTIS [J].
KUROSUMI, M ;
ISHIMURA, K ;
FUJITA, H ;
OSAWA, Y .
HISTOCHEMISTRY, 1985, 83 (05) :401-404
[22]   ROOSTER TESTICULAR GERM-CELLS AND EPIDIDYMAL SPERM CONTAIN P450 AROMATASE [J].
KWON, S ;
HESS, RA ;
BUNICK, D ;
NITTA, H ;
JANULIS, L ;
OSAWA, Y ;
BAHR, JM .
BIOLOGY OF REPRODUCTION, 1995, 53 (06) :1259-1264
[23]   IMMUNOHISTOCHEMICAL EVIDENCE OF THE EXISTENCE AND LOCALIZATION OF AROMATASE IN HUMAN PROSTATIC TISSUES [J].
MATZKIN, H ;
SOLOWAY, MS .
PROSTATE, 1992, 21 (04) :309-314
[24]   GERM-CELLS OF THE MOUSE TESTIS EXPRESS P450 AROMATASE [J].
NITTA, H ;
BUNICK, D ;
HESS, RA ;
JANULIS, L ;
NEWTON, SC ;
MILLETTE, CF ;
OSAWA, Y ;
SHIZUTA, Y ;
TODA, K ;
BAHR, JM .
ENDOCRINOLOGY, 1993, 132 (03) :1396-1401
[25]  
PAYNE A H, 1987, Steroids, V50, P435, DOI 10.1016/0039-128X(87)90030-4
[26]   INTERACTIONS OF FOLLICLE-STIMULATING-HORMONE AND LUTEINIZING-HORMONE IN CONTROLLING ESTRADIOL SYNTHESIS BY THE TESTIS OF THE INFANT RAT [J].
POMERANTZ, DK .
BIOLOGY OF REPRODUCTION, 1983, 28 (04) :890-896
[27]   ONTOGENY OF ESTROGEN SECRETION BY PORCINE FETAL TESTES [J].
RAESIDE, JI ;
WILKINSON, CR ;
FARKAS, G .
ACTA ENDOCRINOLOGICA, 1993, 128 (06) :549-554
[28]  
RIGUADIERE N, 1989, ACTA ENDOCRINOL-COP, V121, P677
[29]  
ROBEL P, 1993, REPRODUCTION MAMMALS, P136
[30]  
ROMMERTS FFG, 1982, J REPROD FERTIL, V65, P281, DOI 10.1530/jrf.0.0650281