The eutD gene of Salmonella enterica encodes a protein with phosphotransacetylase enzyme activity

被引:32
作者
Brinsmade, SR [1 ]
Escalante-Semerena, JC [1 ]
机构
[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53726 USA
关键词
D O I
10.1128/JB.186.6.1890-1892.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The EutD protein of Salmonella enterica is homologous to the catalytic domain of the phosphotransacetylase (Pta) enzyme. The Pta-like activity level of the EutD enzyme compared favorably to that of other Pta enzymes. High-pressure liquid chromatography and mass spectrometry verified that acetyl-coenzyme A was the product of the reaction. The EutD protein restored growth of an S. enterica pta strain on acetate as the source of carbon and energy.
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页码:1890 / 1892
页数:3
相关论文
共 26 条
[11]  
Galperin MY, 2000, PROTEINS, V41, P238, DOI 10.1002/1097-0134(20001101)41:2<238::AID-PROT80>3.0.CO
[12]  
2-L
[13]   PduA is a shell protein of polyhedral organelles involved in coenzyme B12-dependent degradation of 1,2-propanediol in Salmonella enterica serovar typhimurium LT2 [J].
Havemann, GD ;
Sampson, EM ;
Bobik, TA .
JOURNAL OF BACTERIOLOGY, 2002, 184 (05) :1253-1261
[14]   Protein content of polyhedral organelles involved in coenzyme B12-dependent degradation of 1,2-propanediol in Salmonella enterica serovar typhimurium LT2 [J].
Havemann, GD ;
Bobik, TA .
JOURNAL OF BACTERIOLOGY, 2003, 185 (17) :5086-5095
[15]   DETERMINATION OF SHORT-CHAIN ACYL-COENZYME A-ESTERS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
HOSOKAWA, Y ;
SHIMOMURA, Y ;
HARRIS, RA ;
OZAWA, T .
ANALYTICAL BIOCHEMISTRY, 1986, 153 (01) :45-49
[16]   The 17-gene ethanolamine (eut) operon of Salmonella typhimurium encodes five homologues of carboxysome shell proteins [J].
Kofoid, E ;
Rappleye, C ;
Stojiljkovic, I ;
Roth, J .
JOURNAL OF BACTERIOLOGY, 1999, 181 (17) :5317-5329
[17]  
KORNBERG HL, 1966, BIOCHEM J, V99, P1
[18]   CLONING, CHARACTERIZATION, AND FUNCTIONAL EXPRESSION OF ACS, THE GENE WHICH ENCODES ACETYL-COENZYME-A SYNTHETASE IN ESCHERICHIA-COLI [J].
KUMARI, S ;
TISHEL, R ;
EISENBACH, M ;
WOLFE, AJ .
JOURNAL OF BACTERIOLOGY, 1995, 177 (10) :2878-2886
[19]   Regulation of acetyl coenzyme A synthetase in Escherichia coli [J].
Kumari, S ;
Beatty, CM ;
Browning, DF ;
Busby, SJW ;
Simel, EJ ;
Hovel-Miner, G ;
Wolfe, AJ .
JOURNAL OF BACTERIOLOGY, 2000, 182 (15) :4173-4179
[20]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+