An amperometric enzyme electrode for creatine determination prepared by the immobilization of creatinase and sarcosine oxidase in poly(vinylferrocenium)

被引：14

作者：

Erden, PE

Pekyardimci, S

Kilic, E ^{[1
]}

Arslan, F

机构：

[1] Ankara Univ, Fac Sci, Dept Chem, TR-06100 Ankara, Turkey

[2] Gazi Univ, Fac Arts & Sci, Dept Chem, Ankara, Turkey

来源：

ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2006年 / 34卷 / 02期

关键词：

creatine; enzyme electrode; amperometry; poly(vinylferrocen); creatinase; sarcosine oxidase;

D O I：

10.1080/10731190600581775

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new enzyme electrode for the determination of creatine was developed by immobilizing creatinase (CI) and sarcosine oxidase (SO). The enzymes were co-immobilized in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode. Crosslinking with glutaraldehyte (GA) and bovine serum albumin (BSA) was selected as the best immobilization method for the enzymatic system. Determination of creatine was performed by the oxidation of enzymatically generated H2O2 at +0.7 V vs. Ag/AgCl. The linear working range of the electrode was 2.0 x 10(-5) -3.2 x 10(-4) M and the response time was about 50 s. The effects of pH, temperature, enzyme ratio and buffer concentration were investigated and optimum parameters were found to be 7.5, 37 degrees C, 2.5:1 (CI:SO) and 0.05 M, respectively. The stability and reproducibility of the enzyme electrode have been also studied.

引用

页码：223 / 239

页数：17

共 32 条

[11] Miniaturized biosensors employing electropolymerized permselective films and their use for creatinine assays in human serum [J].