Infectious RNA transcripts from grapevine virus a cDNA clone

被引：25

作者：

Galiakparov, N

Tanne, E

Sela, I

Gafny, R ^{[1
]}

机构：

[1] Agr Res Org, Volcani Ctr, Dept Virol, IL-50250 Bet Dagan, Israel

[2] Hebrew Univ Jerusalem, Fac Agr, Otto Warburg Ctr Biotechnol, Virus Lab, IL-76100 Rehovot, Israel

来源：

VIRUS GENES | 1999年 / 19卷 / 03期

关键词：

grapevine virus A; Vitivirus; infectious in vitro transcripts; T7; promoter; dsRNA; rugose wood;

D O I：

10.1023/A:1008192831883

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A full length cDNA clone of grapevine virus A (GVA) was constructed downstream from the bacteriophage T7 RNA polymerase promoter. Capped in vitro-transcribed RNA was infectious in Nicotiana benthamiana and N. clevelandii plants. Symptoms induced by the RNA transcripts or by the parental virus were indistinguishable. The infectivity of the in vitro-transcribed RNA was confirmed by serological detection of the virus coat and movement proteins and by observation of virions by electron microscopy. This is the first report of infectious RNA transcripts derived from a full-length cDNA clone of a member of the Vitivirus genus.

引用

页码：235 / 242

页数：8

共 25 条

[21]

ROSCIGLIONE B, 1983, VITIS, V22, P331

[22] Serological detection of grapevine virus a using antiserum to a nonstructural protein, the putative movement protein [J].