Inkjet-printed polyaniline patterns for exocytosed molecule detection from live cells

被引：13

作者：

Oh, Wan-Kyu ^{[1
]}

Kim, Sojin ^{[1
]}

Shin, Kyoung-Hwan ^{[1
]}

Jang, Yongjin ^{[1
]}

Choi, Moonjung ^{[1
]}

Jang, Jyongsik ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Chem & Biol Engn, WCU, Program Chem Convergence Energy & Environm C2E2, Seoul 151742, South Korea

来源：

TALANTA | 2013年 / 105卷

基金：

新加坡国家研究基金会;

关键词：

Inkjet printing; Conducting polymers; Bioelectronics; Biosensors; Exocytosis; CONDUCTING POLYMERS; RGD PEPTIDES; SENSOR APPLICATIONS; FLEXIBLE SUBSTRATE; ADHESION; POLYMERIZATION; PLASMA; DIFFERENTIATION; NANOMATERIALS; FIBRONECTIN;

D O I：

10.1016/j.talanta.2012.10.050

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Polyaniline (PANi) patterns on flexible substrate are fabricated for biomolecule detection from live cells. PANi patterns are prepared by inkjet printing on polyethylene terephthalate film. Subsequently, arginine-glycine-aspartate (RGD) peptide is immobilized on the PANi pattern to selectively adhere cells. Rat pheochromocytoma PC12 cells are cultured on the RGD-immobilized PANi pattern, and patterned with high selectivity and growth. Additionally, the cells show focal adhesion on the RGD-immobilized PANi pattern, which are confirmed with vinculin staining and scanning electron microscopic images. To monitor dynamic biomolecular release from PC12 cells, RGD-immobilized PANi pattern is used for a real-time electrical signal detector. RGD-immobilized PANi patterning and sensing system represents outstanding ability to translate and amplify exocytosis molecules into a detectable signal as a transducer. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：333 / 339

页数：7

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