A detail study on different aspects of biomolecule immobilization techniques on conducting polymers (CP) for applications in biosensors is described. Comparative studies are conducted in between the different mode of biomolecule immobilization techniques, viz. physical, covalent and electrochemical immobilization onto the conducting polymer films for the fabrication of electrochemical biosensors for clinical, food and environmental monitoring applications. This review focuses on the current status of biomolecule immobilization techniques on CP and their applications in the development of amperometric biosensors. (c) 2006 Elsevier Ltd. All rights reserved.