Structural determinants of ligand binding selectivity between the peroxisome proliferator-activated receptors

被引：464

作者：

Xu, HE ^{[1
]}

Lambert, MH ^{[1
]}

Montana, VG ^{[1
]}

Plunket, KD ^{[1
]}

Moore, LB ^{[1
]}

Collins, JB ^{[1
]}

Oplinger, JA ^{[1
]}

Kliewer, SA ^{[1
]}

Gampe, RT ^{[1
]}

McKee, DD ^{[1
]}

Moore, JT ^{[1
]}

Willson, TM ^{[1
]}

机构：

[1] GlaxoSmithKline, Nucl Receptor Discovery Res, Res Triangle Pk, NC 27709 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 24期

关键词：

D O I：

10.1073/pnas.241410198

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The peroxisome proliferator-activated receptors (PPARs) are transcriptional regulators of glucose, lipid, and cholesterol metabolism. We report the x-ray crystal structure of the ligand binding domain of PPAR alpha (NR1C1) as a complex with the agonist ligand GW409544 and a coactivator motif from the steroid receptor coactivator 1. Through comparison of the crystal structures of the ligand binding domains of the three human PPARs, we have identified molecular determinants of subtype selectivity. A single amino acid, which is tyrosine in PPAR alpha and histidine in PPAR gamma, imparts subtype selectivity for both thiazolidinedione and nonthiazolidinedione ligands. The availability of high-resolution cocrystal structures of the three PPAR subtypes will aid the design of drugs for the treatments of metabolic and cardiovascular diseases.

引用

页码：13919 / 13924

页数：6

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