Equilibrium and irreversible unzipping of DNA in a nanopore

被引：42

作者：

Mathé, J ^{[1
]}

Arinstein, A

Rabin, Y

Meller, A

机构：

[1] Harvard Univ, Rowland Inst, Cambridge, MA 02138 USA

[2] Bar Ilan Univ, Dept Phys, Ramat Gan, Israel

来源：

EUROPHYSICS LETTERS | 2006年 / 73卷 / 01期

关键词：

D O I：

10.1209/epl/i2005-10368-7

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We study the unzipping dynamics of individual DNA hairpins using nanopore force spectroscopy at different voltage ramp rates and temperatures. At high ramp rates the critical unzipping voltage is proportional to log V, where V is the voltage ramp. At low ramp values we observe a crossover to another regime with a weaker dependence on V. Here we report on the dependence of these two regimes on temperature. Remarkably, the unzipping kinetics can be well described by a simple two-states model that predicts the existence of two asymptotic regimes: quasi-equilibrium unzipping at low-voltage ramps and irreversible unzipping at high ramp rates.

引用

页码：128 / 134

页数：7

共 23 条

[1] Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules [J].