Sterol 14-demethylase P450 activity expressed in rat gonads: Contribution to the formation of mammalian meiosis-activating sterol

被引：44

作者：

Yoshida, Y

Yamashita, C

Noshiro, M

Fukuda, M

Aoyama, Y

机构：

[1] HIROSHIMA UNIV, SCH DENT, DEPT BIOCHEM, MINAMI KU, HIROSHIMA 734, JAPAN

[2] SOKA UNIV, FAC ENGN, DEPT BIOENGN, HACHIOJI, TOKYO 192, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 223卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.0929

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sterol 14-demethylase P450 (P45014DM)-dependent formation of 1,4-dimethylcholesta-8,14,23-trienol, that has been reported for a mammalian meiosis-activating sterol, in rat ovaries was confirmed. Transient elevation of ovarian P45014DM activity, that might be related to the female sexual cycle of rat, was observed. The P45014DM activity of immature ovaries was induced by pregnant mare's serum gonadotropin. These bets suggest the contribution of ovarian P45014DM to the gonadotropin-dependent formation of the meiosis-activating sterol, and provide a clue to understanding the mechanism how gonadotropins initiate the resumption of meiosis of mammalian oocytes. It was also found that rat testicular P45014DM participated in the production of 4.4-dimethylcholesta-8,24-dienol that was reported for another meiosis-activating sterol isolated from bull testes. (C) 1996 Academic Press, Inc.

引用

页码：534 / 538

页数：5

共 22 条

[1] DIFFERENT SUBSTRATE SPECIFICITIES OF LANOSTEROL 14-ALPHA-DEMETHYLASE (P-45014DM) OF SACCHAROMYCES-CEREVISIAE AND RAT-LIVER FOR 24-METHYLENE-24,25-DIHYDROLANOSTEROL AND 24,25-DIHYDROLANOSTEROL [J].