Optimized Method for Acinetobacter Species Carbapenemase Detection and Identification by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

被引：38

作者：

Alvarez-Buylla, Adela ^{[1
]}

Picazo, Juan J. ^{[1
]}

Culebras, Esther ^{[1
]}

机构：

[1] Hosp Clin San Carlos, Dept Clin Microbiol, Madrid, Spain

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 05期

关键词：

RESISTANCE; BAUMANNII;

D O I：

10.1128/JCM.00181-13

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for rapid detection and identification of the enzymes responsible for carbapenem resistance in Acinetobacter spp. appears as a promising option, but it will be necessary to have a standardized protocol that facilitates routine use. Based on the results reported herein and comparisons of several previously published reports, we identified the significant peaks for imipenem detection. Optimal bacterial inoculum and incubation time were established, and results obtained with and without dipicolinic acid (DPA) and Zn2+ allowed us to distinguish between metallo-beta-lactamases and oxacillinases.

引用

页码：1589 / 1592

页数：4

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