Whole-rat conditional gene knockout via genome editing

被引：69

作者：

Brown, Andrew J. ^{[1
]}

Fisher, Daniel A. ^{[1
]}

Kouranova, Evguenia ^{[1
]}

McCoy, Aaron ^{[1
]}

Forbes, Kevin ^{[1
]}

Wu, Yumei ^{[1
]}

Henry, Rachel ^{[1
]}

Ji, Diana ^{[1
]}

Chambers, Andre ^{[1
]}

Warren, Joe ^{[1
]}

Shu, Weiguo ^{[1
]}

Weinstein, Edward J. ^{[1
]}

Cui, Xiaoxia ^{[1
]}

机构：

[1] Sage Labs, St Louis, MO USA

来源：

NATURE METHODS | 2013年 / 10卷 / 07期

关键词：

ZINC-FINGER NUCLEASES; EMBRYO MICROINJECTION; TARGETED DISRUPTION; NEURONS; LINES;

D O I：

10.1038/nmeth.2516

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.

引用

页码：638 / +

页数：5

共 17 条

[1]

Bai G, 2009, FRONT NEUROSCI, P79

[2] High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases [J].

Chen, Fuqiang ;

Pruett-Miller, Shondra M. ;

Huang, Yuping ;

Gjoka, Monika ;

Duda, Katarzyna ;

Taunton, Jack ;

Collingwood, Trevor N. ;

Frodin, Morten ;

Davis, Gregory D. .

NATURE METHODS, 2011, 8 (09) :753-U96

[3] Dopaminergic neurons [J].

Chinta, SJ ;

Andersen, JK .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2005, 37 (05) :942-946

[4] Targeted integration in rat and mouse embryos with zinc- finger nucleases [J].

Cui, Xiaoxia ;

Ji, Diana ;

Fisher, Daniel A. ;

Wu, Yumei ;

Briner, David M. ;

Weinstein, Edward J. .

NATURE BIOTECHNOLOGY, 2011, 29 (01) :64-+

[5] TARGETED DISRUPTION OF NMDA RECEPTOR-1 GENE ABOLISHES NMDA RESPONSE AND RESULTS IN NEONATAL DEATH [J].

FORREST, D ;

YUZAKI, M ;

SOARES, HD ;

NG, L ;

LUK, DC ;

SHENG, M ;

STEWART, CL ;

MORGAN, JI ;

CONNOR, JA ;

CURRAN, T .

NEURON, 1994, 13 (02) :325-338

[6] Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases [J].

Geurts, Aron M. ;

Cost, Gregory J. ;

Freyvert, Yevgeniy ;

Zeitler, Bryan ;

Miller, Jeffrey C. ;

Choi, Vivian M. ;

Jenkins, Shirin S. ;

Wood, Adam ;

Cui, Xiaoxia ;

Meng, Xiangdong ;

Vincent, Anna ;

Lam, Stephen ;

Michalkiewicz, Mieczyslaw ;

Schilling, Rebecca ;

Foeckler, Jamie ;

Kalloway, Shawn ;

Weiler, Hartmut ;

Menoret, Severine ;

Anegon, Ignacio ;

Davis, Gregory D. ;

Zhang, Lei ;

Rebar, Edward J. ;

Gregory, Philip D. ;

Urnov, Fyodor D. ;

Jacob, Howard J. ;

Buelow, Roland .

SCIENCE, 2009, 325 (5939) :433-433

[7] Current issues in mouse genome engineering [J].

Glaser, S ;

Anastassiadis, K ;

Stewart, AF .

NATURE GENETICS, 2005, 37 (11) :1187-1193

[8] DELETION OF A DNA-POLYMERASE-BETA GENE SEGMENT IN T-CELLS USING CELL-TYPE-SPECIFIC GENE TARGETING [J].

GU, H ;

MARTH, JD ;

ORBAN, PC ;

MOSSMANN, H ;

RAJEWSKY, K .

SCIENCE, 1994, 265 (5168) :103-106

[9] Creation and preliminary characterization of a Tp53 knockout rat [J].

McCoy, Aaron ;

Besch-Williford, Cynthia L. ;

Franklin, Craig L. ;

Weinstein, Edward J. ;

Cui, Xiaoxia .

DISEASE MODELS & MECHANISMS, 2013, 6 (01) :269-278

[10] CHROMOSOMAL TRANSLOCATIONS IN HUMAN CANCER [J].

RABBITTS, TH .

NATURE, 1994, 372 (6502) :143-149

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