Photoregulation of DNA photolyases in broom Sorghum seedlings

被引:9
作者
Hada, M
Buchholz, G
Hashimoto, T
Nikaido, O
Wellmann, E
机构
[1] Kobe Univ, Fac Sci, Dept Biol, Nada Ku, Kobe, Hyogo 6578501, Japan
[2] Univ Freiburg, Inst Biol 2, Freiburg, Germany
[3] Kobe Womens Univ, Dept Life Sci, Kobe, Hyogo, Japan
[4] Kanazawa Univ, Fac Pharmaceut Sci, Div Radiat Biol, Kanazawa, Ishikawa, Japan
关键词
D O I
10.1111/j.1751-1097.1999.tb03346.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using dark-grown 3 day-old Sorghum bicolor seedlings, photorepair of cyclobutane pyrimidine dimers (CPD) by CPD-photolyase and pyrimidine-(6-4)pyrimidinone photoproducts ([6-4]PP) by [6-4]PP-photolyase was studied in vivo and in vitro. The two types of DNA photoproducts were measured with specific monoclonal antibodies. Sorghum seedlings have different photorepair enzymes for CPD and [6-4]PP. The CPD-photolyase was found to be increased in response to light with maximal efficiency in the UVA/blue spectral range, whereas [6-4]PP-photolyase was not influenced by light.
引用
收藏
页码:681 / 685
页数:5
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