Glucose transporter 8 (GLUT8) from the red imported fire ant, Solenopsis invicta Buren (Hymenoptera: Formicidae)

被引：13

作者：

Chen, Mel-Er ^{[1
]}

Holmes, Steven P. ^{[1
]}

Pietrantonio, Patricia V. ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA

来源：

ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY | 2006年 / 62卷 / 02期

关键词：

insect glucose transporter; transcriptional expression; Drosophila glucose transporter; honey bee GLUT8;

D O I：

10.1002/arch.20119

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned the fire ant glucose transporter 8 (GLUT8) cDNA providing the first molecular characterization of a GLUT8 in insects. Glucose is a poly-alcohol and, due to its high hydrophilicity, cannot move across cell membranes. GLUT8 is a putative facilitative transporter for the cellular import and export of glucose. The complete 2,974-bp cDNA encodes a 501-residue protein with a predicted molecular mass of 54.8 kDa. Transcripts were detected in the brain, midgut, hindgut, Malpighion tubule, fat body, ovary, and testis. The highest transcriptional expression was found in fat body. Northern blot analysis revealed different transcript sizes in mated queen brains, alate female ovaries, and male testes. We propose that four other sequences obtained from insect genome projects from the honey bee Apis mellifera (ENSAPMP00000006624), the malaria mosquito Anopheles gombiae (EAA11842), and the fruit fly Drosophila melanogaster (AAQ23604 and AAM52591) are likely the orthologues of the fire ant GLUT8. Phylogenetic relationships in insect glucose transporters are presented.

引用

页码：55 / 72

页数：18

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