Protein and peptide arrays: Recent trends and new directions

被引：186

作者：

Cretich, M ^{[1
]}

Damin, F ^{[1
]}

Pirri, G ^{[1
]}

Chiari, M ^{[1
]}

机构：

[1] CNR, ICRM, I-20131 Milan, Italy

来源：

BIOMOLECULAR ENGINEERING | 2006年 / 23卷 / 2-3期

关键词：

microarrays; protein arrays; peptide arrays; protcome;

D O I：

10.1016/j.bioeng.2006.02.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microarrays of proteins and peptides make it possible the screening of thousands of binding events in a parallel and high throughput fashion; therefore they are emerging as a powerful tool for proteomics and clinical assays. The complex nature of Proteome, the wide dynamic range of protein concentration in real samples and the critical role of immobilized protein orientation must be taken into account to maximize the utility of protein microarrays. Immobilization strategy and designing of an ideal local chemical environment on the solid surface are both essential for the success of a protein microarray experiment. This review article will focus on protein and peptide arrays highlighting their technical challenges and presenting new directions by means of a set of selected recent applications. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：77 / 88

页数：12

共 103 条

[1] Gains in sensitivity with a device that mixes microarray hybridization solution in a 25-μm-thick chamber [J].