Automation of fluorescence-based PCR for confirmation of meningococcal disease

被引：14

作者：

Diggle, MA ^{[1
]}

Edwards, GFS ^{[1
]}

Clarke, SC ^{[1
]}

机构：

[1] Stobhill Gen Hosp, N Glasgow Univ Hosp NHS Trust, Dept Microbiol, Scottish Meningococcus & Pneumococcus Reference L, Glasgow G21 3UW, Lanark, Scotland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 12期

关键词：

D O I：

10.1128/JCM.39.12.4518-4519.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A fluoresence-based PCR method was developed, fully automated, and used to confirm infection with Neisseria meningitidis by detection of the meningococcus-specific ctrA gene. The method provided a highly sensitive, high-throughput assay that was reproducible and less labor-intensive than manual methods.

引用

收藏

页码：4518 / 4519

页数：2

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