Coenzyme Q is irreplaceable by demethoxy-coenzyme Q in plasma membrane of Caenorhabditis elegans

被引：17

作者：

Arroyo, A ^{[1
]}

Santos-Ocaña, C ^{[1
]}

Ruiz-Ferrer, M ^{[1
]}

Padilla, S ^{[1
]}

Gavilán, A ^{[1
]}

Rodríguez-Aguilera, JC ^{[1
]}

Navas, P ^{[1
]}

机构：

[1] Univ Pablo Olavide, Ctr Andaluz Biol Desarrollo, E-41013 Seville, Spain

来源：

FEBS LETTERS | 2006年 / 580卷 / 07期

关键词：

coenzyme Q; demethoxy-coenzyme Q; plasma membrane; clk-1 mutant phenotype; Caenorhabditis elegans;

D O I：

10.1016/j.febslet.2006.02.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure was developed to isolate fractions enriched in plasma membrane from Caenorhabditis elegans. Coenzyme Q(9) (Q(9)) was found in plasma membrane isolated from either wild-type or long-lived qm30 and qm51 clk-1 mutant strains of Caenorhabditis elegans, along with dietary coenzyme Q(8) (Q(8)) and the biosynthetic intermediate demethoxy-Q(9) (DMQ(9)). NADH was able to reduce both Q(8) and Q(9), but not DMQ(9). Our results indicate that DMQ(9) cannot achieve the same redox role of Q(9) in plasma membrane, suggesting that proportion of all these Q isoforms in plasma membrane must be an important factor in establishing the clk-1 mutant phenotype. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1740 / 1746

页数：7

共 40 条

[1] Silencing of ubiquinone biosynthesis genes extends life span in Caenorhabditis elegans [J].

Asencio, C ;

Rodríguez-Aguilera, JC ;

Ruiz-Ferrer, M ;

Vela, J ;

Navas, P .

FASEB JOURNAL, 2003, 17 (06) :1135-+

[2] The Saccharomyces cerevisiae COQ10 gene encodes a START domain protein required for function of coenzyme Q in respiration [J].

Barros, MH ;

Johnson, A ;

Gin, P ;

Marbois, BN ;

Clarke, CF ;

Tzagoloff, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (52) :42627-42635

[3]

Branicky R, 2000, BIOESSAYS, V22, P48, DOI 10.1002/(SICI)1521-1878(200001)22:1&lt

[4]

48::AID-BIES9&gt

[5]

3.0.CO

[6]

2-F

[7] A proposed mechanism for the lowering of mitochondrial electron leak by caloric restriction [J].

de Grey, ADNJ .

MITOCHONDRION, 2001, 1 (02) :129-139

[8] CLK-1 controls respiration, behavior and aging in the nematode Caenorhabditis elegans [J].

Felkai, S ;

Ewbank, JJ ;

Lemieux, J ;

Labbé, JC ;

Brown, GG ;

Hekimi, S .

EMBO JOURNAL, 1999, 18 (07) :1783-1792

[9] Coenzyme Q distribution in HL-60 human cells depends on the endomembrane system [J].

Fernández-Ayala, DJM ;

Brea-Calvo, G ;

López-Lluch, G ;

Navas, P .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2005, 1713 (02) :129-137

[10] A ubiquinone-binding site regulates the mitochondrial permeability transition pore [J].

Fontaine, E ;

Ichas, F ;

Bernardi, P .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (40) :25734-25740

← 1 2 3 4 →