Coenzyme Q is irreplaceable by demethoxy-coenzyme Q in plasma membrane of Caenorhabditis elegans

被引：17

作者：

Arroyo, A ^{[1
]}

Santos-Ocaña, C ^{[1
]}

Ruiz-Ferrer, M ^{[1
]}

Padilla, S ^{[1
]}

Gavilán, A ^{[1
]}

Rodríguez-Aguilera, JC ^{[1
]}

Navas, P ^{[1
]}

机构：

[1] Univ Pablo Olavide, Ctr Andaluz Biol Desarrollo, E-41013 Seville, Spain

来源：

FEBS LETTERS | 2006年 / 580卷 / 07期

关键词：

coenzyme Q; demethoxy-coenzyme Q; plasma membrane; clk-1 mutant phenotype; Caenorhabditis elegans;

D O I：

10.1016/j.febslet.2006.02.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure was developed to isolate fractions enriched in plasma membrane from Caenorhabditis elegans. Coenzyme Q(9) (Q(9)) was found in plasma membrane isolated from either wild-type or long-lived qm30 and qm51 clk-1 mutant strains of Caenorhabditis elegans, along with dietary coenzyme Q(8) (Q(8)) and the biosynthetic intermediate demethoxy-Q(9) (DMQ(9)). NADH was able to reduce both Q(8) and Q(9), but not DMQ(9). Our results indicate that DMQ(9) cannot achieve the same redox role of Q(9) in plasma membrane, suggesting that proportion of all these Q isoforms in plasma membrane must be an important factor in establishing the clk-1 mutant phenotype. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1740 / 1746

页数：7

共 40 条

[21]

JONASSEN T, 2001, COENZYME Q MOL MECH, P185

[22] Plasma membrane NADH-coenzyme Q0 reductase generates semiquinone radicals and recycles vitamin E homologue in a superoxide-dependent reaction [J].

Kagan, VE ;

Arroyo, A ;

Tyurin, VA ;

Tyurina, YY ;

Villalba, JM ;

Navas, P .

FEBS LETTERS, 1998, 428 (1-2) :43-46

[23]

Lagendijk J, 1996, J LIPID RES, V37, P67

[24] Extension of life-span in Caenorhabditis elegans by a diet lacking coenzyme Q [J].

Larsen, PL ;

Clarke, CF .

SCIENCE, 2002, 295 (5552) :120-123

[25] Quinone specificity of Complex I [J].

Lenaz, G .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1364 (02) :207-221

[26] Ubiquinone is necessary for mouse embryonic development but is not essential for mitochondrial respiration [J].

Levavasseur, F ;

Miyadera, H ;

Sirois, J ;

Tremblay, ML ;

Kita, K ;

Shoubridge, E ;

Hekimi, S .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (49) :46160-46164

[27] Selective induction of apoptosis by capsaicin in transformed cells:: the role of reactive oxygen species and calcium [J].

Macho, A ;

Calzado, MA ;

Muñoz-Blanco, J ;

Gómez-Díaz, C ;

Gajate, C ;

Mollinedo, F ;

Navas, P ;

Muñoz, E .

CELL DEATH AND DIFFERENTIATION, 1999, 6 (02) :155-165

[28] Quinones in long-lived clk-1 mutants of Caenorhabditis elegans [J].

Miyadera, H ;

Kano, K ;

Miyoshi, H ;

Ishii, N ;

Hekimi, S ;

Kita, K .

FEBS LETTERS, 2002, 512 (1-3) :33-37

[29] Altered quinone biosynthesis in the long-lived clk-1 mutants of Caenorhabditis elegans [J].

Miyadera, H ;

Amino, H ;

Hiraishi, A ;

Taka, H ;

Murayama, K ;

Miyoshi, H ;

Sakamoto, K ;

Ishii, N ;

Hekimi, S ;

Kita, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (11) :7713-7716

[30] Mouse homologue of coq7/clk-1, longevity gene in Caenorhabditis elegans, is essential for coenzyme Q synthesis, maintenance of mitochondrial integrity, and neurogenesis [J].

Nakai, D ;

Yuasa, S ;

Takahashi, M ;

Shimizu, T ;

Asaumi, S ;

Isono, K ;

Takao, T ;

Suzuki, Y ;

Kuroyanagi, H ;

Hirokawa, K ;

Koseki, H ;

Shirsawa, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 289 (02) :463-471

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