Surfactant protein D enhances bacterial antigen presentation by bone marrow-derived dendritic cells

被引:90
作者
Brinker, KG
Martin, E
Borron, P
Mostaghel, E
Doyle, C
Harding, CV
Wright, JR
机构
[1] Duke Univ, Med Ctr, Dept Cell Biol, Durham, NC 27710 USA
[2] Duke Univ, Med Ctr, Dept Immunol, Durham, NC 27710 USA
[3] Duke Univ, Med Ctr, Dept Med, Durham, NC 27710 USA
[4] Case Western Reserve Univ, Inst Pathol, Cleveland, OH 44106 USA
关键词
antigen presenting cell; innate immune response; adaptive immune response; phagocytosis; lung;
D O I
10.1152/ajplung.2001.281.6.L1453
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Surfactant protein (SP) D functions as a soluble pattern recognition molecule to mediate the clearance of pathogens by phagocytes in the innate immune response. We hypothesize that SP-D may also interact with dendritic cells, the most potent antigen presenting cell, to enhance uptake and presentation of bacterial antigens. Using mouse bone marrow-derived dendritic cells, we show that SP-D binds to immature dendritic cells in a dose-, carbohydrate-, and calcium-dependent manner, whereas SP-D binding to mature dendritic cells is reduced. SP-D also binds to Escherichia coli HB101 and enhances its association with dendritic cells. Additionally, SP-D enhances the antigen presentation of an ovalbumin fusion protein expressed in E. coli HB101 to ovalbumin-specific major histocompatibility complex class II T cell hybridomas. The enhancement of antigen presentation by SP-D is dose dependent and is not shared by other collectin-like proteins tested. These studies demonstrate that SP-D augments antigen presentation by dendritic cells and suggest that innate immune molecules such as SP-D may help initiate an adaptive immune response for the purpose of resolving an infection.
引用
收藏
页码:L1453 / L1463
页数:11
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