Supporting conditional mouse mutagenesis with a comprehensive cre characterization resource

被引：186

作者：

Heffner, Caleb S. ^{[1
]}

Pratt, C. Herbert ^{[1
]}

Babiuk, Randal P. ^{[1
]}

Sharma, Yashoda ^{[2
]}

Rockwood, Stephen F. ^{[1
]}

Donahue, Leah R. ^{[1
]}

Eppig, Janan T. ^{[1
]}

Murray, Stephen A. ^{[1
]}

机构：

[1] Jackson Lab, Bar Harbor, ME 04609 USA

[2] Yale Univ, Sch Med, New Haven, CT 06520 USA

来源：

NATURE COMMUNICATIONS | 2012年 / 3卷

关键词：

FLUORESCENT PROTEIN; IN-VIVO; EXPRESSION; GENE; RECOMBINASE; CELLS; MICE; LINE; TOOL; MANIPULATION;

D O I：

10.1038/ncomms2186

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Full realization of the value of the loxP-flanked alleles generated by the International Knockout Mouse Consortium will require a large set of well-characterized cre-driver lines. However, many cre driver lines display excision activity beyond the intended tissue or cell type, and these data are frequently unavailable to the potential user. Here we describe a high-throughput pipeline to extend characterization of cre driver lines to document excision activity in a wide range of tissues at multiple time points and disseminate these data to the scientific community. Our results show that the majority of cre strains exhibit some degree of unreported recombinase activity. In addition, we observe frequent mosaicism, inconsistent activity and parent-of-origin effects. Together, these results highlight the importance of deep characterization of cre strains, and provide the scientific community with a critical resource for cre strain information.

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页数：9

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