Transformation of Saccharomyces cerevisiae with a cDNA encoding a sterol C-methyltransferase from Arabidopsis thaliana results in the synthesis of 24-ethyl sterols

被引:45
作者
Husselstein, T
Gachotte, D
Desprez, T
Bard, M
Benveniste, P
机构
[1] INST BOT,DEPT BIOL CELLULAIRE & MOLEC,INST BIOL MOLEC PLANTES,F-67083 STRASBOURG,FRANCE
[2] INDIANA UNIV PURDUE UNIV,INDIANAPOLIS,IN 46202
[3] INRA,BIOL CELLULAIRE LAB,F-78026 VERSAILLES,FRANCE
关键词
Arabidopsis; S-adenosylmethionine-sterol-C-methyltransferase; erg6; complementation;
D O I
10.1016/0014-5793(96)00089-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using an EST-cDNA probe, a full-length cDNA (411) sequence of 1411 bp was isolated from A. thaliana. This sequence contained features typical of methyltransferases in general and in particular showed 38% identity with ERG6, a S. cerevisiae gene which encodes the zymosterol-C-24-methyltransferase, A yeast vector containing this ORF (4118-pYeDP60) was used to transform a mild type S. cerevisiae which accumulates predominantly ergosterol, a 24-methyl sterol as well as a mutant erg6 null mutant accumulating principally zymosterol, a sterol non-alkylated at C-24, In both cases, several 24-ethyl- and 24-ethylidene sterols were synthetized indicating that the 4118 cDNA encodes a plant sterol C-methyltransferase able to perform two sequential methylations of the sterol side chain.
引用
收藏
页码:87 / 92
页数:6
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