CLONING, DISRUPTION AND SEQUENCE OF THE GENE ENCODING YEAST C-5 STEROL DESATURASE

被引：128

作者：

ARTHINGTON, BA

BENNETT, LG

SKATRUD, PL

GUYNN, CJ

BARBUCH, RJ

ULBRIGHT, CE

BARD, M

机构：

[1] INDIANA UNIV PURDUE UNIV,DEPT BIOL,1125 E 38 ST,INDIANAPOLIS,IN 46205

[2] VET AFFAIRS MED CTR,INDIANAPOLIS,IN 46202

[3] ELI LILLY & CO,LILLY RES LAB,INDIANAPOLIS,IN 46285

[4] MERRELL DOW PHARMACEUT INC,RES INST,CINCINNATI,OH 45215

来源：

GENE | 1991年 / 102卷 / 01期

关键词：

ERGOSTEROL BIOSYNTHESIS; SPARKING FUNCTION; RECOMBINANT DNA;

D O I：

10.1016/0378-1119(91)90535-J

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The ERG3 gene from Saccharomyces cerevisiae has been cloned by complementation of an erg3-2 mutation. ERG3 is the putative gene encoding the C-5 sterol desaturase required for ergosterol biosynthesis. The functional gene has been localized on a 2.5-kb HindIII-BamHI fragment containing an open reading frame comprising 365 amino acids. Gene disruption resulting from a deletion/substitution demonstrates that ERG3 is not essential for cell viability or the sparking function.

引用

页码：39 / 44

页数：6

共 20 条

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