Attenuated BDNF-induced upregulation of GABAergic markers in neurons lacking Xbp1

被引：39

作者：

Hayashi, Akiko ^{[1
]}

Kasahara, Takaoki ^{[1
]}

Kametani, Mizue ^{[1
]}

Kato, Tadafumi ^{[1
]}

机构：

[1] RIKEN Brain Sci Inst, Lab Mol Dynam Mental Disorders, Wako, Saitama 3510198, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 376卷 / 04期

关键词：

Xbp1; Endoplasmic reticulum stress; Unfolded protein response; Somatostatin; Neuropeptide Y; Calbindin; Bipolar disorder; Schizophrenia; Gamma-aminobutyric acid;

D O I：

10.1016/j.bbrc.2008.09.059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

XBP1 is a transcription factor induced by unconventional splicing associated with endoplasmic reticulum, stress and plays a role in development. Brain-derived neurotrophic factor (BDNF) causes splicing of Xbp1 mRNA in neurites, and Xbp1 is required for BDNF-induced neurite extension and branching. To search for the molecular mechanisms of how Xbp1 Plays a role in neural development, comprehensive gene expression analysis was performed in primary telencephalic neurons obtained from Xbp1 knockout mice at embryonic day 12.5. By searching for the genes induced by BDNF in wild type neurons but not in Xbp1 knockout mice, we found that upregulation of three GABAergic markers, somatostatin (Sst), neuropeptide Y (Npy), and calbindin (Calb1), were compromised in Xbp1 knockout neurons. Attenuated upregulation of Npy and Calb1 in Xbp1 knockout neurons was confirmed by quantitative RT-PCR. This finding may be relevant to impaired BDNF-induced neurite extension in Xbp1 knockout neurons. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：758 / 763

页数：6

共 28 条

[1] XBP1 controls diverse cell type- and condition-specific transcriptional regulatory networks [J].

Acosta-Alvear, Diego ;

Zhou, Yiming ;

Blais, Alexandre ;

Tsikitis, Mary ;

Lents, Nathan H. ;

Arias, Carolina ;

Lennon, Christen J. ;

Kluger, Yuval ;

Dynlacht, Brian David .

MOLECULAR CELL, 2007, 27 (01) :53-66

[2] Reduction of nonpyramidal cells in sector CA2 of schizophrenics and manic depressives [J].

Benes, FM ;

Kwok, EW ;

Vincent, SL ;

Todtenkopf, MS .

BIOLOGICAL PSYCHIATRY, 1998, 44 (02) :88-97

[3] Regulation of the GABA cell phenotype in hippocampus of schizophrenics and bipolars [J].

Benes, Francine M. ;

Lim, Benjamin ;

Matzilevich, David ;

Walsh, John P. ;

Subburaju, Sivan ;

Minns, Martin .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (24) :10164-10169

[4] Lack of support for a genetic association of the XBP1 promoter polymorphism with bipolar disorder in probands of European origin [J].

Cichon, S ;

Buervenich, S ;

Kirov, G ;

Akula, N ;

Dimitrova, A ;

Green, E ;

Schumacher, J ;

Klopp, N ;

Becker, T ;

Ohlraun, S ;

Schulze, TG ;

Tullius, M ;

Gross, MM ;

Jones, L ;

Krastev, S ;

Nikolov, I ;

Hamshere, M ;

Jones, I ;

Czerski, PM ;

Leszczynska-Rodziewicz, A ;

Kapelski, P ;

Van Den Bogaert, A ;

Illig, T ;

Hauser, J ;

Maier, W ;

Berrettini, W ;

Byerley, W ;

Coryell, W ;

Gershon, ES ;

Kelsoe, JR ;

McInnis, MG ;

Murphy, DL ;

Nurnberger, JI ;

Reich, T ;

Scheftner, W ;

O'Donovan, MC ;

Propping, P ;

Owen, MJ ;

Rietschel, M ;

Nöthen, MM ;

McMahon, FJ ;

Craddock, N .

NATURE GENETICS, 2004, 36 (08) :783-784

[5] The basic domain leucine zipper protein hXBP-1 preferentially binds to and transactivates CRE-like sequences containing an ACGT core [J].

Clauss, IM ;

Chu, M ;

Zhao, JL ;

Glimcher, LH .

NUCLEIC ACIDS RESEARCH, 1996, 24 (10) :1855-1864

[6] Serum brain-derived neurotrophic factor is decreased in bipolar disorder during depressive and manic episodes [J].

Cunha, Angelo B. M. ;

Frey, Benicio N. ;

Andreazza, Ana C. ;

Goi, Julia D. ;

Rosa, Adriane R. ;

Goncalves, Carlos A. ;

Santin, Aida ;

Kapczinski, Flavio .

NEUROSCIENCE LETTERS, 2006, 398 (03) :215-219

[7] Specificity and timing of neocortical transcriptome changes in response to BDNF gene ablation during embryogenesis or adulthood [J].

Glorioso, C. ;

Sabatini, M. ;

Unger, T. ;

Hashimoto, T. ;

Monteggia, L. M. ;

Lewis, D. A. ;

Mirnics, K. .

MOLECULAR PSYCHIATRY, 2006, 11 (07) :633-648

[8] A role for the unfolded protein response in optimizing antibody secretion [J].

Gunn, KE ;

Gifford, NM ;

Mori, K ;

Brewer, JW .

MOLECULAR IMMUNOLOGY, 2004, 41 (09) :919-927

[9] The role of brain-derived neurotrophic factor (BDNF)- induced XBP1 splicing during brain development [J].

Hayashi, Akiko ;

Kasahara, Takaoki ;

Iwamoto, Kazuya ;

Ishiwata, Mizuho ;

Kametani, Mizue ;

Kakiuchi, Chihiro ;

Furuichi, Teiichi ;

Kato, Tadafumi .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (47) :34525-34534

[10] TARGETED DISRUPTION OF THE BDNF GENE PERTURBS BRAIN AND SENSORY NEURON DEVELOPMENT BUT NOT MOTOR-NEURON DEVELOPMENT [J].

JONES, KR ;

FARINAS, I ;

BACKUS, C ;

REICHARDT, LF .

CELL, 1994, 76 (06) :989-999

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