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RNA diversity has profound effects on the translation of neuronal nitric oxide synthase

被引：160
作者：
Wang, Y
Newton, DC
Robb, GB
Kau, CL
Miller, TL
Cheung, AH
Hall, AV
VanDamme, S
Wilcox, JN
Marsden, PA
机构：
[1] St Michaels Hosp, Div Renal, Toronto, ON M4X 1B1, Canada
[2] St Michaels Hosp, Dept Med, Toronto, ON M4X 1B1, Canada
[3] Univ Toronto, Toronto, ON M4X 1B1, Canada
[4] Emory Univ, Dept Med, Div Hematol, Atlanta, GA 30322 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 21期
关键词：
D O I：
10.1073/pnas.96.21.12150
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A comprehensive analysis of the structure of neuronal nitric oxide synthase (nNOS; EC 1.14.13.39) mRNA species revealed NOS1 to be the most structurally diverse human gene described to date in terms of promoter usage. Nine unique exon 1 variants are variously used for transcript initiation in diverse tissues, and each is expressed from a unique 5'-flanking region. The dependence on unique genomic regions to control transcription initiation in a cell-specific fashion burdens the transcripts with complex 5'-mRNA leader sequences. Elaborate splicing patterns that involve alternatively spliced leader exons and exon skipping have been superimposed on this diversity. Highly structured nNOS mRNA 5'-untranslated regions, which have profound effects on translation both in vitro and in cells, contain cis RNA elements that modulate translational efficiency in response to changes in cellular phenotype.
引用
收藏
页码：12150 / 12155
页数：6
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