Spectrofluorimetric study of the inclusion complex of 7-hydroxymethylnalidixic acid with gamma-cyclodextrin in aqueous solution

被引：15

作者：

DuranMeras, I

DelaPena, AM

Salinas, F

Caceres, IR

机构：

[1] Department of Analytical Chemistry, University of Extremadura

来源：

APPLIED SPECTROSCOPY | 1997年 / 51卷 / 05期

关键词：

7-hydroxymethylnalidixic acid; gamma-cyclodextrin; spectrofluorimetry;

D O I：

10.1366/0003702971940828

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A fluorimetric study on the spectral characteristics and acid-base behavior of 7-hydroxymethylnalidixic acid has been performed, The host-guest complexation process between gamma-cyclodextrin and 7-hydroxymethylnalidixic acid has also been investigated by fluorescence spectroscopy. Scatchard and Benesi-Hildebrand methods have been applied to determine the stoichiometry of the complex, and a 1:1 stoichiometric ratio has been established. A K-1 = 118 +/- 25 M-1 formation constant has been calculated for the inclusion complex, by using the changes produced on the fluorescence of 7-hydroxymethylnalidixic acid, when it is included on the hydrophobic cyclodextrin cavity. A fluorimetric method of determination of 7-hydroxy- methylnalidixic acid, in gamma-cyclodextrin aqueous solution, has been proposed. The analytical parameters and detection and quantification limits of the method have been determined.

引用

页码：684 / 688

页数：5

共 33 条

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