Podoplanin, a novel marker of tumor-initiating cells in human squamous cell carcinoma A431

被引：113

作者：

Atsumi, Naho ^{[1
,2
]}

Ishii, Genichiro ^{[1
]}

Kojima, Motohiro ^{[1
]}

Sanada, Masaru ^{[1
,2
]}

Fujii, Satoshi ^{[1
]}

Ochiai, Atsushi ^{[1
,2
]}

机构：

[1] Natl Canc Ctr Hosp E, Div Pathol, Res Ctr Innovat Oncol, Chiba 2778577, Japan

[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Canc Biol Lab, Chiba, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 373卷 / 01期

关键词：

podoplanin; squamous cell carcinoma; tumor-initiating cells; cancer stem cells; CD44; sonic hedgehog;

D O I：

10.1016/j.bbrc.2008.05.163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Squamous cell carcinoma (SCC) is a malignant tumor that shows morphologic and phenotypic similarities to normally differentiated squamous epithelium. Thus, it may be an ideal model for seeking a marker of tumor-initiating cells (TICs) based on their morphology. Using the human SCC cell line A431, we found that, as a paradigm of cancer stem cells: (1) podoplanin(+) cells generate both podoplanin(+) and podoplanin cells; (2) podoplanin(-) cells rarely generate podoplanin(+) cells: (3) podoplanin(+) cells have higher colony formation efficiency and tumorigenicity than podoplanin- cells; (4) localization and morphology of podoplanin(+) cells in a xenografted tumor derived from podoplanin(+) cells are similar with those in human oral SCC tissue or normal epithelium. Furthermore, podoplanin(+) A431 cells share sonic hedgehog and CD44 expression with stem cells in normal squamous epithelium. Hence, we concluded that podoplanin is a novel marker to enrich TICs with stem-cell-like properties from SCC cell line A431. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：36 / 41

页数：6

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